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N-Acetylglucosamine (O-linked) monoclonal antibody (RL2)

 
ALX-804-111-R100 100 µl 594.00 USD
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Product Specification

Alternative Name:Glc-NAc (O-linked)
 
Clone:RL2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Pore complex-lamina fraction purified from rat liver nuclear envelopes.
 
Species reactivity:Human, Mouse, Rat
 
Specificity:By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC.
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections, 1:200)
Western Blot (1:1,000). Do not use with diluents containing glycosylated proteins
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1: G. Majumdar, et al.; Diabetes 53, 3184 (2004), Abstract; Full Text
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins: C.M. Snow, et al.; J. Cell. Biol. 104, 1143 (1987), Abstract;

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N-Acetylglucosamine
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