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α-Catenin monoclonal antibody (15D9)

 
ALX-804-101-C100 100 µg 443.00 USD
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Product Specification

Clone:15D9
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human α-catenin.
 
UniProt ID:P35221 (α1), P26232 (α2), Q9UI47 (α3)
 
Species reactivity:Human, Mouse, Rat
Dog
 
Crossreactivity:Does not cross-react with β-catenins.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated)
Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. ALX-840-026)
 
Purity Detail:Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
 
Formulation:Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
 
Reconstitution:Reconstitute with 1ml distilled water (15 min., RT).
 
Use/Stability:Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

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