Product Details
| Alternative Name: | MR |
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| Clone: | H10E4C9F |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Aldosterone. |
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| UniProt ID: | P08235 (human), P22199 (rat) |
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| Source: | Produced using the anti-idiotypic method. |
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| Species reactivity: | Human, Rat
Chicken, Rabbit
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| Applications: | ICC, IHC (PS), WB, FUNC
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1:100; staining is blocked by pre-incubating the sample with aldosterone)
Western Blot (1:1,000; ECL: Enhanced detection systems like enhanced chemiluminescence are recommended).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~116kDa by Western blot.
Functional Application: Blocks the binding of aldosterone to the MR.
Not suitable for immunoprecipitation. |
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. Purified ascites in PBS containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Pituitary expression of type I and type II glucocorticoid receptors during chicken embryonic development and their involvement in growth hormone cell differentiation: I. Bossis, et al.; Endocrinology
145, 3523 (2004),
Abstract;
Full Text
Identification of glucocorticoid receptor domains involved in transrepression of transforming growth factor-beta action: G. Li, et al.; J. Biol. Chem.
278, 41779 (2003),
Abstract;
Full Text
The mineralocorticoid receptor may compensate for the loss of the glucocorticoid receptor at specific stages of mammary gland development: M. Kingsley-Kallesen, et al.; Mol. Endocrinol.
16, 2008 (2002),
Abstract;
Full Text
Prerequisite for cardiac aldosterone action. Mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in the human heart: M. Lombes, et al.; Circulation
92, 175 (1995),
Abstract;
Full Text
Aldosterone antagonists destabilize the mineralocorticosteroid receptor: B. Couette, et al.; Biochem. J.
282, 697 (1992),
Abstract;
Immunohistochemical and biochemical evidence for a cardiovascular mineralocorticoid receptor: M. Lombes, et al.; Circ. Res.
71, 503 (1992),
Abstract;
Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone: M. Lombes, et al.; PNAS
87, 1086 (1990),
Abstract;
Full Text
Internal image properties of a monoclonal auto-anti-idiotypic antibody and its binding to aldosterone receptors: M. Lombes, et al.; J. Biol. Chem.
264, 2528 (1989),
Abstract;
Full Text
