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trans-Golgi network 38 monoclonal antibody (2F7.1)

 
ALX-804-095-R100 100 µl 585.00 USD
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Product Specification

Alternative Name:TGN38
 
Clone:2F7.1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 1-17 (L1PSASKPNNTS
SENNPP17) of N-terminal rat TGN38 (trans-Golgi network 38).
 
UniProt ID:P19814
 
Species reactivity:Human, Rat
Monkey
 
Specificity:Recognizes the luminal domain of TGN38.
 
Applications:Flow Cytometry, ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~38kDa by Western blot, representing deglycosylated TGN38. Under normal Western blot conditions detects a band of ~85-95kDa depending on cell type.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The trans-Golgi network (TGN) is part of the secretory pathway of eukaryotic cells which is distinct from the Golgi stack.The TGN is important in the later stages of protein secretion where it seems to play a key role in the sorting and targeting of secreted proteins to the correct destination. Brefeldin A (Prod. No. BML-G405) causes the contents of the Golgi stack to be redistributed into the endoplasmatic reticulum while the TGN collapse upon the microtubule organizing center. This makes immunofluorescence detection of TGN38, following Brefeldin A treatment, a useful tool for the discrimination of the TGN from the Golgi stack.
 
804-095
Figure: Immunofluorescence staining of TGN38 (red) with MAb to trans-Golgi Network 38 (2F7.1) (Prod. No. ALX-804-095) and β-COP (green) with PAb to β-COP (Pro. No. ALX-210-127) in NRK cells.
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804-095

Product Literature References

Bst-2/HM1.24 is a raft-associated apical membrane protein with an unusual topology: S. Kupzig, et al.; Traffic 4, 694 (2003), Abstract; Full Text
GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat: K. Kawamoto, et al.; Traffic 3, 483 (2002), Abstract; Full Text
Efficient trafficking of TGN38 from the endosome to the trans-Golgi network requires a free hydroxyl group at position 331 in the cytosolic domain: E.P. Roquemore and G. Banting; Mol. Biol. Cell 9, 2125 (1998), Abstract; Full Text
Lumenal and transmembrane domains play a role in sorting type I membrane proteins on endocytic pathways: B.J. Reaves, et al.; Mol. Biol. Cell 9, 1107 (1998), Abstract; Full Text
Okadaic acid treatment leads to fragmentation of the trans-Golgi network and an increase of TGN38 at the cell surface: M. Horn & G. Banting; Biochem. J. 301, 69 (1994), Abstract;

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