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United States 

Phospholamban monoclonal antibody (2D12)

 
ALX-804-093-R100 100 µl 578.00 USD
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The antibody can also be used to increase calcium uptake in ventricular SR vesicle analogous to the phosphorylation of phospholamban following adrenergic stimulation.

Product Specification

Alternative Name:PLB
 
Clone:2D12
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 2-25 (D2KVQYLTRSAIRRASTIEMPQQAR25) of dog PLB (phospholamban).
 
UniProt ID:P61012
 
Species reactivity:Human, Mouse, Rat
Chicken, Dog, Guinea pig, Porcine, Rabbit, Sheep
 
Specificity:Recognizes an epitope between aa 9-17 of dog PLB.
 
Applications:ICC, IHC (PS), IP, WB, FUNC
 
Recommended Dilutions/Conditions:Immunocytochemistry (20µg/ml)
Western Blot (2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~25kDa (non-reducing conditions; representing the pentameric form of PLB) and ~5kDa (reducing conditions; representing the monomeric form of PLB) by Western blot.
Functional Application: increases the calcium uptake in ventricular SR vesicles.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Chronic ventricular myocyte-specific overexpression of angiotensin II type 2 receptor results in intrinsic myocyte contractile dysfunction: M. Nakayama, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H317 (2005), Abstract; Full Text
Differential activation of stress-response signaling in load-induced cardiac hypertrophy and failure: B.A. Rothermel, et al.; Physiol. Genomics 23, 18 (2005), Abstract; Full Text
Genetic manipulation of calcium-handling proteins in cardiac myocytes. I. Experimental studies: P. Coutu and J.M. Metzger; Am. J. Physiol. Heart Circ. Physiol. 288, H601 (2005), Abstract; Full Text
Adenylyl cyclase type VI gene transfer reduces phospholamban expression in cardiac myocytes via activating transcription factor 3: M.H. Gao, et al.; J. Biol. Chem. 279, 38797 (2004), Abstract; Full Text
Alterations in myofilament function contribute to left ventricular dysfunction in pigs early after myocardial infarction: J. van der Velden, et al.; Circ. Res. 95, e85 (2004), Abstract; Full Text
Autologous stem cell transplantation for myocardial repair: J. Liu, et al.; Am. J. Physiol. Heart Circ. Physiol. 287, H501 (2004), Abstract; Full Text
Doxycycline inducible expression of SERCA2a improves calcium handling and reverts cardiac dysfunction in pressure overload-induced cardiac hypertrophy: J. Suarez, et al.; Am. J. Physiol. Heart Circ. Physiol. 287, H2164 (2004), Abstract; Full Text
Frequency- and afterload-dependent cardiac modulation in vivo by troponin I with constitutively active protein kinase A phosphorylation sites: E. Takimoto, et al.; Circ. Res. 94, 496 (2004), Abstract; Full Text
Sarcoplasmic reticulum calcium defect in Ras-induced hypertrophic cardiomyopathy heart: M. Zheng, et al.; Am. J. Physiol. Heart Circ. Physiol. 286, H424 (2004), Abstract; Full Text
The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm: T.L. Radzyukevich, et al.; Am. J. Physiol. Cell Physiol. 287, C1300 (2004), Abstract; Full Text
Mechanism of enhanced cardiac function in mice with hypertrophy induced by overexpressed Akt: Y.K. Kim, et al.; J. Biol. Chem. 278, 47622 (2003), Abstract; Full Text
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation: K.Y. Xu, et al.; J. Biol. Chem. 278, 41798 (2003), Abstract; Full Text
Regional alterations in protein expression in the dyssynchronous failing heart: D.D. Spragg, et al.; Circulation 108, 929 (2003), Abstract; Full Text
Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17: Y. Ji, et al.; J. Biol. Chem. 278, 25063 (2003), Abstract; Full Text
Abnormal Ca2+ release, but normal ryanodine receptors, in canine and human heart failure: M.T. Jiang, et al.; Circ. Res. 91, 1015 (2002), Abstract; Full Text
Altered dose response to beta-agonists in SERCA1a-expressing hearts ex vivo and in vivo: S. Huke, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H958 (2002), Abstract; Full Text
Ca(2+) signaling in cardiac myocytes overexpressing the alpha(1) subunit of L-type Ca(2+) channel: L.S. Song, et al.; Circ. Res. 90, 174 (2002), Abstract; Full Text
Defective intracellular Ca(2+) signaling contributes to cardiomyopathy in Type 1 diabetic rats: K.M. Choi, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H1398 (2002), Abstract; Full Text
Interaction between increased SERCA2a activity and beta -adrenoceptor stimulation in adult rabbit myocytes: B. Chaudhri, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H2450 (2002), Abstract; Full Text
Overexpression of the sarcoplasmic reticulum Ca(2+)-ATPase improves myocardial contractility in diabetic cardiomyopathy: S.U. Trost, et al.; Diabetes 51, 1166 (2002), Abstract; Full Text
Targeting phospholamban by gene transfer in human heart failure: F. del Monte, et al.; Circulation 105, 904 (2002), Abstract; Full Text
Type 1 phosphatase, a negative regulator of cardiac function: A.N. Carr, et al.; Mol. Cell. Biol. 22, 4124 (2002), Abstract; Full Text
Mechanisms of altered excitation-contraction coupling in canine tachycardia-induced heart failure, I: experimental studies: B. O’Rourke, et al.; Circ. Res. 84, 562 (1999), Abstract; Full Text
Molecular and physiological alterations in murine ventricular dysfunction: H.A. Rockman, et al.; PNAS 91, 2694 (1994), Abstract; Full Text
Comparative studies of cardiac and skeletal sarcoplasmic reticulum ATPases. Effect of a phospholamban antibody on enzyme activation by Ca2+: T. Cantilina, et al.; J. Biol. Chem. 268, 17018 (1993), Abstract; Full Text
Residues 2-25 of phospholamban are insufficient to inhibit Ca2+ transport ATPase of cardiac sarcoplasmic reticulum: L.R. Jones and L.J. Field; J. Biol. Chem. 268, 11486 (1993), Abstract; Full Text
The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle: A.O. Jorgensen, et al.; J. Cell Biol. 120, 969 (1993), Abstract; Full Text
Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase: F.N. Briggs, et al.; J. Biol. Chem. 267, 26056 (1992), Abstract; Full Text
Phospholamban mediates the b-adrenergic-enhanced Ca2+ uptake in mammalian ventricular myocytes: J.S.K. Sham, et al.; Am. J. Physiol. 261, H1344 (1991), Abstract;

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