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H+/K+ ATPase-β monoclonal antibody (2G11)

 
ALX-804-084-R100 100 µl 410.00 USD
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Product Specification

Alternative Name:Potassium-transporting ATPase subunit β
 
Clone:2G11
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified 34kDa core peptide from deglycosylated pig gastric microsomes.
 
UniProt ID:P18434 (pig), P51164 (human)
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Ferret, Porcine, Rabbit
 
Specificity:Recognizes the β subunit of H+/K+ ATPase.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB, FUNC
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:2,000)
Immunohistochemistry (paraffin and frozen sections, 1:2,000)
Western Blot (1:4,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: Inhibits enzymatic activity of H+/ K+ ATPase and alters affinity of its cytoplasmic potassium binding site.
 
Formulation:Liquid. PBS-diluted ascites containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Localization and function of soluble N-ethylmaleimide-sensitive factor attachment protein-25 and vesicle-associated membrane protein-2 in functioning gastric parietal cells: S. Karvar, et al.; J. Biol. Chem. 277, 50030 (2002), Abstract; Full Text
Functionally distinct pools of actin in secretory cells: D.A. Ammar, et al.; Am. J. Physiol. Cell Physiol. 281, C407 (2001), Abstract; Full Text
Expression of rab11a N124I in gastric parietal cells inhibits stimulatory recruitment of the H+-K+-ATPase: J.G. Duman, et al.; Am. J. Physiol. 277, C361 (1999), Abstract; Full Text

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