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ERK1/2 monoclonal antibody (12D4)

 
ALX-804-061-C100 100 µg 528.00 USD
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Product Specification

Alternative Name:MAPK2/3, MAP kinase 2/3
 
Clone:12D4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2 conjugated to KLH.
 
UniProt ID:P27361 (Erk1), P28482 (Erk2)
 
Species reactivity:Human, Mouse, Rat
Dog
 
Specificity:Recognizes the pThr-Glu-pTyr motif of activated ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein.
 
Crossreactivity:Does not cross-react with activated JNK or p38.
 
Applications:ELISA, ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1-10µg/ml)
Immunoprecipitation (1-10µg per 106 pervanadate-treated A431 or HepG2 cells)
Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. ALX-840-025)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Replacement for ADI-905-651
 

Product Literature References

IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005), Abstract; Full Text

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