United States
β-Catenin (NT/exon 2), mAb (7D11)
| ALX-804-060-C100 | 100 µg | 431.00 USD |  |
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Replaces Prod. #: BML-BA1711
Product Specification
| Purity Detail: | Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography. |
| Formulation: | Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose. |
| Clone: | 7D11 |
| Isotype: | Mouse IgG2a |
| Immunogen: | Recombinant human β-catenin. |
| Reconstitution: | Reconstitute with 1ml distilled water (15 min., RT). |
| Specificity: | Recognizes the N-terminus (exon 2) of human, mouse, rat (weak) and dog β-catenin. |
| Application: | ELISA Immunoprecipitation Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. |
| Long Term Storage: | -20°C |
| Use/Stability: | Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
| Handling: | After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles. |
| Positive Control: | Included (Prod. No. ALX-840-027). |
Figure A: Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies.
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
Figure B: Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies.
Method: β-catenin probes were applied to SDS-PAGE, transferred to PVDF membranes, probed for 1 hour at 15-22°C with the indicated MAbs (0.5µg/ml) and developed with ECL (exposure time 5 sec.). Antibodies used are:
Lane 1: MAb to β-Catenin (dephospho, aa 35-50) (7A7) (Prod. No. ALX-804-259)
Lane 2: MAb to β-Catenin (NT/Exon 2) (7D11) (Prod. No. ALX-804-060)
Lane 3: MAb to β-Catenin (dephospho, aa 27-37) (8E4) (Prod. No. ALX-804-260)
Lane 4: MAb to β-Catenin (Exon 3) (9G2) (Prod. No. ALX-804-058)
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Product Literature References
Phospho-beta-catenin accumulation in Alzheimer's disease and in aggresomes attributable to proteasome dysfunction: M. Ghanevati & C.A. Miller; J. Mol. Neurosci. 25, 79 (2005), Abstract;
The human F box protein beta-Trcp associates with the Cul1/Skp1 complex and regulates the stability of beta-catenin: E. Latres, et al.; Oncogene 18, 849 (1999), Abstract;
Abnormal immunoreactivity of the E-cadherin-catenin complex in gastric carcinoma: relationship with patient survival: A. Jawhari, et al.; Gastroenterology 112, 46 (1997), Abstract;
beta-catenin is a target for the ubiquitin-proteasome pathway: H. Aberle, et al.; Embo J. 16, 3797 (1997), Abstract;
Beta-catenin mutations in cell lines established from human colorectal cancers: M. lyas, et al.; Proc. Natl. Acad. Sci. USA 94, 10330 (1997), Abstract;
Functional interaction of beta-catenin with the transcription factor LEF-1: J. Behrens, et al.; Nature 382, 638 (1996), Abstract;
XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos: M. Molenaar, et al.; Cell 86, 391 (1996), Abstract;
Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein: S. Munemitsu, et al.; Proc. Natl. Acad. Sci. USA 92, 3046 (1995), Abstract;
Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor: H. Hoschuetzky, et al.; J. Cell Biol. 127, 1375 (1994), Abstract;
Responses to Wnt signals in vertebrate embryos may involve changes in cell adhesion and cell movement: R.T. Moon, et al.; J. Cell Sci. Suppl. 17, 183 (1993), Abstract;
The product of the Drosophila segment polarity gene armadillo is part of a multi-protein complex resembling the vertebrate adherens junction: M. Peifer; J. Cell Sci. 105, 993 (1993), Abstract;
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