Product Specification
| Clone: | CB5.4 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant mouse perforin (aa 98-534). |
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| UniProt ID: | P10820 |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes an epitope in the region aa 402-534. |
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| Applications: | ICC
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C. |
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| Handling: | Do not freeze. Protect from light. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Technical Info/Product Notes: | 1 test means: 1µl of a 1mg/ml stock solution were used to stain 200’000 cells in a sample volume of 50µl. |
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Schematic structure of mouse perforin (recombinant, aa. 98-534). The antibody recognizes an epitope within aa. 402-534.
Flow cytometry analysis of 106 Jurkat cells using Perforin (mouse) Monoclonal Antibody (CB5.4), DyLight™488 Conjugate at a concentration of 50 μg/mL
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Product Literature References
Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol.
173, 3660 (2004),
Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem.
277, 15904 (2002),
Abstract;
Full Text
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol.
153, 2470 (1994),
Abstract;
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med.
177, 1 (1993),
Abstract;
General Literature References
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol.
173, 3660 (2004),
Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem.
277, 15904 (2002),
Abstract;
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