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Perforin (mouse) monoclonal antibody (CB5.4)

 
ALX-804-057-C100 100 µg Purified 301.00 USD
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Product Specification

Clone:CB5.4
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse perforin (aa 98-534).
 
UniProt ID:P10820
 
Species reactivity:Mouse
 
Specificity:Recognizes an epitope in the region aa 402-534.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-057
Schematic structure of mouse perforin (recombinant, aa. 98-534). The antibody recognizes an epitope within aa. 402-534.
804-057-1
Figure: Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species.
Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
804-057-2
Figure: Detection of perforin in an individual mouse T cell. Perforin is located in cytoplasmic granules.
Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 µg/ml of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC).
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Product Literature References

Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract; Full Text
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol. 153, 2470 (1994), Abstract;
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993), Abstract;

General Literature References

Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract;

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Perforin (mouse) monoclonal antibody (CB5.4) (FITC conjugate) 

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