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Perforin (mouse) monoclonal antibody (CB5.4)

 
ALX-804-057-C100 100 µg Purified 316.00 USD
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Product Specification

Clone:CB5.4
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse perforin (aa 98-534).
 
UniProt ID:P10820
 
Source:Purified from mouse ascites
 
Species reactivity:Mouse
 
Specificity:Recognizes an epitope in the region aa 402-534.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Perforin (mouse) monoclonal antibody (CB5.4) image
Figure: Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species.
Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
Perforin (mouse) monoclonal antibody (CB5.4) (FITC conjugate) Schematic structure of immunogen
Schematic structure of mouse perforin (recombinant, aa. 98-534). The antibody recognizes an epitope within aa. 402-534.
Perforin (mouse) monoclonal antibody (CB5.4) image
Figure: Detection of perforin in an individual mouse T cell. Perforin is located in cytoplasmic granules.
Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 µg/ml of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC).
Perforin (mouse) monoclonal antibody (CB5.4) Western blot
Western Blot analysis of Perforin (mouse) mAb (CB5.4) (Prod. No. ALX-804-057): Lane 1: MW Marker, Lane 2: CTLL-2 cell lysate, Lane 3: 3T3 cell lysate. Western Blot analysis of Perforin 1 expression in CTLL-2 cell lysate and absence of perforin in the mouse fibroblast cell line 3T3.
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Perforin (mouse) monoclonal antibody (CB5.4) image Perforin (mouse) monoclonal antibody (CB5.4) (FITC conjugate) Schematic structure of immunogen Perforin (mouse) monoclonal antibody (CB5.4) image Perforin (mouse) monoclonal antibody (CB5.4) Western blot

Product Literature References

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation: D. Wang, et al.; Immunity 48, 659 (2018), Abstract;
Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery: T. Clemente, et al.; Cell Death Dis. 8, e3176 (2017), Abstract; Full Text
Pentoxifylline reverses chronic experimental Chagasic cardiomyopathy in association with repositioning of abnormal CD8+ T-cell response: I.R. Pereira, et al.; PLoS Negl. Trop. Dis. 9, e0003659 (2015), Abstract; Full Text
Tumor necrosis factor is a therapeutic target for immunological unbalance and cardiac abnormalities in chronic experimental Chagas' heart disease: I.R. Pereira, et al.; Mediators Inflamm. 2014, 798078 (2014), Abstract; Full Text
Th cells promote CTL survival and memory via acquired pMHC-I and endogenous IL-2 and CD40L signaling and by modulating apoptosis-controlling pathways: C.S. Umeshappa, et al.; PLoS One 8, e64787 (2013), Abstract; Full Text
The Rac activator DOCK2 regulates natural killer cell-mediated cytotoxicity in mice through the lytic synapse formation: Y. Sakai, et al.; Blood 122, 386 (2013), Abstract;
An islet-specific pulse of TGF-β abrogates CTL function and promotes β cell survival independent of Foxp3+ T cells: M. Wallberg, et al.; J. Immunol. 186, 2543 (2011), Abstract;
Perforin-expressing cytotoxic cells contribute to chronic cardiomyopathy in Trypanosoma cruzi infection: J.C. Silverio, et al.; Int. J. Exp. Pathol. 91, 72 (2010), Abstract; Full Text
Switch from perforin-expressing to perforin-deficient CD8(+) T cells accounts for two distinct types of effector cytotoxic T lymphocytes in vivo: A. Meiraz, et al.; Immunology 128, 69 (2009), Abstract; Full Text
A new murine model to define the critical pathologic and therapeutic mediators of polymyositis: T. Sugihara, et al.; Arthritis Rheum. 56, 1304 (2007), Abstract;
Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice: H. Takahashi, et al.; Vaccine 24, 3591 (2006), Abstract;
Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract; Full Text
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol. 153, 2470 (1994), Abstract;
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993), Abstract;

General Literature References

Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract;

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