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Perforin (mouse) monoclonal antibody (CB5.4)

ALX-804-057-C100 100 µg Purified 316.00 USD
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Product Specification

Immunogen:Recombinant mouse perforin (aa 98-534).
UniProt ID:P10820
Source:Purified from mouse ascites
Species reactivity:Mouse
Specificity:Recognizes an epitope in the region aa 402-534.
Applications:ICC, IHC (FS), IHC (PS), IP, WB
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Schematic structure of mouse perforin (recombinant, aa. 98-534). The antibody recognizes an epitope within aa. 402-534.
Figure: Detection of perforin in the mouse T cell clone B6.1. (lane 1). Absence of perforin in the mouse fibroblast cell line NIH.3T3 (lane 2). The protein migrates as a 66 kDa species.
Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
Figure: Detection of perforin in an individual mouse T cell. Perforin is located in cytoplasmic granules.
Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 µg/ml of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC).
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Product Literature References

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation: D. Wang, et al.; Immunity 48, 659 (2018), Abstract;
Proteomic and functional analysis identifies galectin-1 as a novel regulatory component of the cytotoxic granule machinery: T. Clemente, et al.; Cell Death Dis. 8, e3176 (2017), Abstract; Full Text
Pentoxifylline reverses chronic experimental Chagasic cardiomyopathy in association with repositioning of abnormal CD8+ T-cell response: I.R. Pereira, et al.; PLoS Negl. Trop. Dis. 9, e0003659 (2015), Abstract; Full Text
Tumor necrosis factor is a therapeutic target for immunological unbalance and cardiac abnormalities in chronic experimental Chagas' heart disease: I.R. Pereira, et al.; Mediators Inflamm. 2014, 798078 (2014), Abstract; Full Text
Th cells promote CTL survival and memory via acquired pMHC-I and endogenous IL-2 and CD40L signaling and by modulating apoptosis-controlling pathways: C.S. Umeshappa, et al.; PLoS One 8, e64787 (2013), Abstract; Full Text
The Rac activator DOCK2 regulates natural killer cell-mediated cytotoxicity in mice through the lytic synapse formation: Y. Sakai, et al.; Blood 122, 386 (2013), Abstract;
An islet-specific pulse of TGF-β abrogates CTL function and promotes β cell survival independent of Foxp3+ T cells: M. Wallberg, et al.; J. Immunol. 186, 2543 (2011), Abstract;
Perforin-expressing cytotoxic cells contribute to chronic cardiomyopathy in Trypanosoma cruzi infection: J.C. Silverio, et al.; Int. J. Exp. Pathol. 91, 72 (2010), Abstract; Full Text
Switch from perforin-expressing to perforin-deficient CD8(+) T cells accounts for two distinct types of effector cytotoxic T lymphocytes in vivo: A. Meiraz, et al.; Immunology 128, 69 (2009), Abstract; Full Text
A new murine model to define the critical pathologic and therapeutic mediators of polymyositis: T. Sugihara, et al.; Arthritis Rheum. 56, 1304 (2007), Abstract;
Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice: H. Takahashi, et al.; Vaccine 24, 3591 (2006), Abstract;
Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract; Full Text
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol. 153, 2470 (1994), Abstract;
The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993), Abstract;

General Literature References

Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract;

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