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μ-Calpain (domain III) monoclonal antibody (9A4H8D3)

 
ALX-804-050-R100 100 µl 594.00 USD
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Replaces Prod. #: BML-SA257

This antibody has been successfully used in Western blot procedures to detect µ-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, and rat myoblasts, kidney, liver and spleen.  Immunofluorescence staining of µ-calpain in porcine LLC-PK1 cells with ALX-804-050 results in diffuse cytoplasmic staining.

Product Specification

Alternative Name:Calpain I
 
Clone:9A4H8D3
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified bovine µ-calpain.
 
UniProt ID:Q27970
 
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Porcine, Rabbit
 
Specificity:Recognizes an epitope in domain III (aa 465-520) of µ-calpain from several mammalian sources (e.g. human, mouse, rat, pig, bovine, hamster, rabbit).
 
Crossreactivity:Does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
 
Applications:ICC, IHC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:2,000)
Note: Not recommended for immunoprecipitation.
 
Application Notes:Detects a band of ~80kDa by Western blot.
 
Formulation:Liquid. Ascites containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Calpain is a major cell death effector in selective striatal degeneration induced in vivo by 3-nitropropionate: implications for Huntington’s disease: N. Bizat, et al.; J. Neurosci. 23, 5020 (2003), Abstract; Full Text
Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25: G. Kusakawa, et al.; J. Biol. Chem. 275, 17166 (2000), Abstract; Full Text
Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains: J. Cong, et al.; J. Biol. Chem. 268, 25740 (1993), Abstract; Full Text
A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells: R.D. Lane, et al.; Exp. Cell Res. 203, 5 (1992), Abstract;

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