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PMCA ATPase monoclonal antibody (5F10)

 
ALX-804-049-R100 100 µl 439.00 USD
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Product Specification

Alternative Name:Plasma membrane calcium pump ATPase
 
Clone:5F10
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Purified human erythrocyte PMCA ATPase (plasma membrane calcium pump ATPase).
 
UniProt ID:Q00804 (PMCA1), Q01814 (PMCA2)
 
Species reactivity:Human, Mouse, Rat
Amphibian, Cat, Chicken, Dog, Monkey, Rabbit, Sheep
 
Specificity:Recognizes all known (currently four) isoforms of plasma membrane PMCA ATPase. Recognizes an epitope between aa 724-783 of human PMCA ATPase.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:500)
Immunohistochemistry (paraffin sections, 1:500)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~140kDa by Western blot. Also detects bands at ~95kDa and ~180kDa which may represent aggregation and/or natural proteolytic products of PMCA ATPase.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta: E.E. Champion, et al.; Placenta 24, 453 (2003), Abstract;
Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies: A.G. Filoteo, et al.; J. Biol. Chem. 272, 23741 (1997), Abstract; Full Text
Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules: J.L. Borke, et al.; Am. J. Physiol. 257, F842 (1989), Abstract;

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