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Nitric oxide synthase (universal) monoclonal antibody (NOS-3F7-B11-B5)

 
ALX-804-029-R200 200 µl 399.00 USD
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Product Specification

Alternative Name:NOS (universal)
 
Clone:NOS-3F7-B11-B5
 
Host:Mouse
 
Isotype:IgM
 
Immunogen:Purified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain.
 
UniProt ID:E1BKI3
 
Species reactivity:Mouse, Rat
Bovine
 
Specificity:Recognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I).
 
Applications:IHC (FS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation: K.Y. Xu, et al.; J. Biol. Chem. 278, 41798 (2003), Abstract; Full Text
Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia: C. Xue, et al.; Am. J. Physiol. 267, L667 (1994), Abstract;

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