Product Details
| Alternative Name: | NFATc1, Nuclear factor of activated T cells, cytoplasmic 1, NFAT2, NFATC |
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| Clone: | 7A6 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654. |
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| UniProt ID: | O95644 |
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| Species reactivity: | Human, Mouse, Rat
Monkey
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| Crossreactivity: | Does not cross-react with NF-ATc2. |
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| Applications: | ICC, IF, IHC, IP, WB
ChIP, Gel Shift
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:250)
Immunofluorescence (1:10-1:100)
Immunohistochemistry (1:20-200)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~105kDa by Western blot. |
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| Formulation: | Liquid. Ascites diluted in PBS containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Note: Confusion may arise regarding NFAT protein versus gene names. According to UniProt, the nomenclature is as follows:Nuclear Factor of Activated T Cells, Cytoplasmic 1 (NF-ATc1) Gene Names: NFATC1 or NFATC or NFAT2
Nuclear Factor of Activated T Cells, Cytoplasmic 2 (NF-ATc2) Gene Names: NFATC2 or NFATP or NFAT1
Nuclear Factor of Activated T Cells, Cytoplasmic 3 (NF-ATc3) Gene Names: NFATC3 or NFATX or NFAT4
Nuclear Factor of Activated T Cells, Cytoplasmic 4 (NF-ATc4) Gene Names: NFATC4 or NFAT3
Nuclear Factor of Activated T Cells (NF-AT5) Gene Names: NFAT5 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Activity- and calcineurin-independent nuclear shuttling of NFATc1, but not NFATc3, in adult skeletal muscle fibers: T. Shen, et al.; Mol. Biol. Cell.
17, 1570 (2006),
Abstract;
Full Text
Proteolytic regulation of nuclear factor of activated T (NFAT) c2 cells and NFAT activity by caspase-3: W. Wu, et al.; J. Biol. Chem.
281, 10682 (2006),
Abstract;
Full Text
Blockade of nuclear factor of activated T cells activation signaling suppresses balloon injury-induced neointima formation in a rat carotid artery model: Z. Liu, et al.; J. Biol. Chem.
280, 14700 (2005),
Abstract;
Full Text
Calcium-dependent activation of interleukin-21 gene expression in T cells: H.P. Kim, et al.; J. Biol. Chem.
280, 25291 (2005),
Abstract;
Full Text
Rocaglamide derivatives are immunosuppressive phytochemicals that target NF-AT activity in T cells: P. Proksch, et al.; J. Immunol.
174, 7075 (2005),
Abstract;
Full Text
Vasopressin-dependent myogenic cell differentiation is mediated by both Ca2+/calmodulin-dependent kinase and calcineurin pathways: B.M. Scicchitano, et al.; Mol. Biol. Cell
16, 3632 (2005),
Abstract;
Full Text
Active protein kinase B regulates TCR responsiveness by modulating cytoplasmic-nuclear localization of NFAT and NF-kappa B proteins: A.K. Patra, et al.; J. Immunol.
172, 4812 (2004),
Abstract;
Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells: A. Kerstan & T. Hunig; J. Immunol.
172, 1341 (2004),
Abstract;
Differential roles for Wiskott-Aldrich syndrome protein in immune synapse formation and IL-2 production: J.L. Cannon and J.K. Burkhardt; J. Immunol.
173, 1658 (2004),
Abstract;
Full Text
IL-4-Induced GATA-3 Expression Is a Time-Restricted Instruction Switch for Th2 Cell Differentiation: N. Seki, et al.; J. Immunol.
172, 6158 (2004),
Abstract;
Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms: J. Barlic, et al.; J. Biol. Chem.
279, 48520 (2004),
Abstract;
Full Text
NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching: K.J. McCullagh, et al.; PNAS
101, 10590 (2004),
Abstract;
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Role of NFAT proteins in IL13 gene transcription in mast cells: S. Monticelli, et al.; J. Biol. Chem.
279, 36210 (2004),
Abstract;
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Vascular endothelial growth factor- and thrombin-induced termination factor, Down syndrome critical region-1, attenuates endothelial cell proliferation and angiogenesis: T. Minami, et al.; J. Biol. Chem.
279, 50537 (2004),
Abstract;
Full Text
Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells: C. Pfeifhofer, et al.; J. Exp. Med.
197, 1525 (2003),
Abstract;
Full Text
Cannabinol enhancement of interleukin-2 (IL-2) expression by T cells is associated with an increase in IL-2 distal nuclear factor of activated T cell activity: T.R. Jan, et al.; Mol. Pharmacol.
61, 446 (2002),
Abstract;
Full Text
Induction of NFATc2 expression by interleukin 6 promotes T helper type 2 differentiation: S. Diehl, et al.; J. Exp. Med.
196, 39 (2002),
Abstract;
Full Text
The basis for TCR-mediated regulation of the IL-2 receptor alpha chain gene: role of widely separated regulatory elements: H.P. Kim & W.J. Leonard; EMBO J.
21, 3051 (2002),
Abstract;
Full Text
The calcineurin-NFAT pathway and muscle fiber-type gene expression: S.J. Swoap, et al.; Am. J. Physiol. Cell Physiol.
279, C915 (2000),
Abstract;
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Expression of NFAT-family proteins in normal human T cells: L. Lyakh, et al.; Mol. Cell. Biol.
17, 2475 (1997),
Abstract;
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Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells: K.M. Latinis, et al.; J. Biol. Chem.
272, 31427 (1997),
Abstract;
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NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity: S.N. Ho, et al.; J. Biol. Chem.
270, 19898 (1995),
Abstract;
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