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Ryanodine receptor monoclonal antibody (34C)

 
ALX-804-016-R100 100 µl 615.00 USD
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Replaces Prod. #: BML-SA245

Product Specification

Alternative Name:RyR
 
Clone:34C
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Partially purified chicken pectoral muscle RyR (ryanodine receptor).
 
UniProt ID:Q90984
 
Species reactivity:Human, Mouse, Rat
Amphibian, Bovine, Dog, Fish, Mink, Monkey, Rabbit, Sheep
 
Specificity:Recognizes RyR-1 and RyR-2 isoforms. In chicken, it detects the α, β and cardiac isoforms; in frog the α and β isoforms; and in fish the α isoform. Also recognizes RyR-3 in mouse cells.
 
Applications:ICC, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:1,000)
Western Blot (1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~565kDa by Western blot, representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565kDa, representing the α and β isoforms.
 
Formulation:Liquid. Contains 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function: R.A. Moore, et al.; J. Cell. Biol. 140, 843 (1998), Abstract;
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle: F. Protasi, et al.; J. Cell. Biol. 140, 831 (1998), Abstract;
Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms: E.B. Olivares, et al.; Biophys. J. 59, 1153 (1991), Abstract;
Ryanodine and inositol triphosphate receptors coexist in avian cerebellar Purkinje neurons: P.D. Walton, et al.; J. Cell. Biol. 113, 1145 (1991), Abstract;
Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle: J.A. Airey, et al.; J. Biol. Chem. 265, 14187 (1990), Abstract; Full Text

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