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Ryanodine receptor monoclonal antibody (C3-33)

 
ALX-804-015-C100 100 µg 604.00 USD
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Replaces Prod. #: BML-SA244

Product Specification

Alternative Name:RyR
 
Clone:C3-33
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Dog cardiac RyR (ryanodine receptor).
 
Species reactivity:Human, Mouse, Rat
Amphibian, Chicken, Dog, Fish, Guinea pig, Rabbit
 
Crossreactivity:Cross-reacts weakly with RyR-1.
 
Applications:Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:50)
Immunohistochemistry (frozen sections, 1µg/ml)
Immunohistochemistry (paraffin sections, 1µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of 565kDa by Western blot. In non-mammalian vertebrates, a doublet is seen at 565kDa representing the α and β isoforms of the receptor.
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
 
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Figure: Immunolocalization of ryanodine receptor 1 (RyR1) in rat brain hippocampus using Monoclonal Antibody to Ryanodine Receptor (C3-33) (Prod. No. ALX-804-015).
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Product Literature References

cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells: A.N. Yusufi, et al.; Am. J. Physiol. Renal Physiol. 281, F91 (2001), Abstract; Full Text
Functional characterization of mutants in the predicted pore region of the rabbit cardiac muscle Ca(2+) release channel (ryanodine receptor isoform 2):: G.G. Du, et al.; J. Biol. Chem. 276, 31760 (2001), Abstract; Full Text
Colocalization of dihydropyridine and ryanodine receptors in neonate rabbit heart using confocal microscopy: F. Sedarat, et al.; Am. J. Physiol. Heart Circ. Physiol. 279, H202 (2000), Abstract; Full Text
Expression and functional characterization of the cardiac muscle ryanodine receptor Ca(2+) release channel in Chinese hamster ovary cells: M.B. Bhat, et al.; Biophys. J. 77, 808 (1999), Abstract; Full Text
Extended junctional sarcoplasmic reticulum of avian cardiac muscle contains functional ryanodine receptors: J. Junker, et al.; J. Biol. Chem. 269, 1627 (1994), Abstract; Full Text
Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle: F.A. Lai, et al.; Am. J. Physiol. 263, C365 (1992), Abstract;

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