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PDI monoclonal antibody (RL90)

 
ALX-804-012-R100 100 µl 585.00 USD
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Product Specification

Alternative Name:Protein disulfide-isomerase
 
Clone:RL90
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Purified rat PDI (protein disulfide-isomerase).
 
UniProt ID:P04785
 
Species reactivity:Human, Mouse, Rat
Hamster, Porcine
 
Applications:Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB, FUNC
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100)
Immunohistochemistry (frozen sections, 1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~59kDa (rat liver extract) or ~61kDa (human liver extract) by Western blot.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. Purified antibody containing 1mg/ml BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
 
Technical Info/Product Notes:Immunohistochemical staining of PDI in rat intestine with Prod. No. ALX-804-012 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures, Prod. No. ALX-804-012 has been shown to inhibit the activity of PDI in vitro. Prod. No. ALX-804-012 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.
 

Product Literature References

Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase: S. Baumann & T. Hennet; J. Biol. Chem. 291, 18514 (2016), Application(s): Immunofluorescence, Abstract; Full Text
Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology 478C, 75 (2015), Application(s): Immunofluorescence, Abstract;
Sustained integrin ligation involves extracellular free sulfhydryls and enzymatically catalyzed disulfide exchange: J. Lahav, et al.; Blood 100, 2472 (2002), Abstract; Full Text
Inhibition of human immunodeficiency virus infection by agents that interfere with thiol-disulfide interchange upon virus-receptor interaction: H.J. Ryser, et al.; PNAS 91, 4559 (1994), Abstract; Full Text
Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase: R. Mandel, et al.; PNAS 90, 4112 (1993), Abstract; Full Text
Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies: C.S. Kaetzel, et al.; Biochem. J. 241, 39 (1987), Abstract;

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