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FasL (mouse) monoclonal antibody (H11) (FITC conjugate)

ALX-804-010F-T100 100 tests FITC 432.00 USD
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Product Specification

Alternative Name:CD178, CD95L, APO-1L, TNFSF 6
Immunogen:Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
UniProt ID:P41047
Species reactivity:Mouse
Applications:Flow Cytometry, ICC
Application Notes:For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009). For the detection of human FasL by flow cytometry or Western blot, we recommmend MAb to FasL (human) (Mike-2) (Prod. No. ALX-804-125).
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Technical Info/Product Notes:1 test means: 1µl of a 1mg/ml stock solution were used to stain 200'000 cells in a sample volume of 50µl.
Schematic structure of mouse FasL (peptide, aa 196-220)
Figure: Flow cytometric profile of 293T cells transiently transfected with a FasL-expression plasmid (filled profile). The open profile corresponds to mock-transfected 293T cells.
Method: FasL-transfected 293T cells (5 x 105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg of FITC-labelled H11 antibody. After washing in FACS buffer, cells were anlyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition (see T. Renno, M. Hahne, J. Tschopp and H. R. MacDonald, J. Exp. Med., 183, 431 (1996)).
Note: Activated primary T cells do not express high levels of surface FasL!

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804-010 804-010-1

Product Literature References

Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001), Abstract;
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997), Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;

General Literature References

Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997), Abstract;
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995), Abstract;
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995), Abstract;
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994), Abstract;
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90, 4409 (1993), Abstract; Full Text

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