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FasL (mouse) monoclonal antibody (H11) (biotin conjugate)

 
ALX-804-010B-T100 100 tests Biotin 373.00 USD
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Product Specification

Alternative Name:CD178, CD95L, APO-1L, TNFSF 6
 
Clone:H11
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
 
UniProt ID:P41047
 
Species reactivity:Mouse
 
Applications:Flow Cytometry, ICC, WB
 
Application Notes:For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009). For the detection of human FasL by flow cytometry or Western blot, we recommmend MAb to FasL (human) (Mike-2) (Prod. No. ALX-804-125).
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
FasL (mouse) monoclonal antibody (H11) (biotin conjugate) image
Figure: Detection of FasL in 293T cells transfected with a FasL expression vector.
Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 µg/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope.
FasL (mouse) monoclonal antibody (H11) (biotin conjugate) Schematic structure of immunogen
Schematic structure of mouse FasL (peptide, aa 196-220)
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FasL (mouse) monoclonal antibody (H11) (biotin conjugate) image FasL (mouse) monoclonal antibody (H11) (biotin conjugate) Schematic structure of immunogen

Product Literature References

Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001), Abstract;
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997), Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;

General Literature References

Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997), Abstract;
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995), Abstract;
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995), Abstract;
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994), Abstract;
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90, 4409 (1993), Abstract; Full Text

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