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FasL (mouse) monoclonal antibody (H11) (biotin conjugate)

 
ALX-804-010B-T100 100 tests Biotin 345.00 USD
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Product Specification

Alternative Name:CD178, CD95L, APO-1L, TNFSF 6
 
Clone:H11
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
 
UniProt ID:P41047
 
Species reactivity:Mouse
 
Applications:Flow Cytometry, ICC, WB
 
Application Notes:For immunohistochemistry, we recommend MAb to FasL (A11) (Prod. No. ALX-804-009). For the detection of human FasL by flow cytometry or Western blot, we recommmend MAb to FasL (human) (Mike-2) (Prod. No. ALX-804-125).
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
804-010
Schematic structure of mouse FasL (peptide, aa 196-220)
804-010-3
Figure: Detection of FasL in 293T cells transfected with a FasL expression vector.
Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 µg/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope.
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Product Literature References

Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts: L. Tourneur, et al.; J. Immunol. 167, 1338 (2001), Abstract;
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL: J.K. Wang, et al.; Cell. Immunol. 179, 153 (1997), Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;

General Literature References

Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997), Abstract;
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995), Abstract;
Fas ligand-induced apoptosis as a mechanism of immune privilege: T.S. Griffith, et al.; Science 270, 1189 (1995), Abstract;
Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways: B. Lowin, et al.; Nature 370, 650 (1994), Abstract;
Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: H. Russell, et al.; PNAS 90, 4409 (1993), Abstract; Full Text

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