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FasL (mouse) monoclonal antibody (A11)

 
ALX-804-009-C100 100 µg 296.00 USD
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Product Specification

Alternative Name:CD178, CD95L, APO-1L, TNFSF 6
 
Clone:A11
 
Host:Rat
 
Isotype:IgM
 
Immunogen:Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L; APO-1L; CD178).
 
UniProt ID:P41047
 
Species reactivity:Mouse
 
Applications:Flow Cytometry, IHC (FS), IHC (PS)
 
Application Notes:For Western blot, we recommend MAb to FasL (mouse) (H11) (Prod. No. ALX-804-010), or MAb to FasL (human) (Mike-2) (Prod. No. ALX-804-125).
 
Formulation:Liquid. Purified antibody in PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
FasL (mouse) monoclonal antibody (A11) Schematic structure of immunogen
Schematic structure of mouse FasL (peptide, aa. 196-220)
FasL (mouse) monoclonal antibody (A11) Immunohistochemistry
Figure: Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody.
Method: Cryostat sections (6-8µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 µg/ml of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
Paraffin sections: Dewax in xylene (2x10 min), EtOH 100% (2x10 min), block endogeneous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/ml antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC)."
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FasL (mouse) monoclonal antibody (A11) Schematic structure of immunogen FasL (mouse) monoclonal antibody (A11) Immunohistochemistry

Product Literature References

Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions: I. Viard-Leveugle, et al.; J. Biol. Chem. 278, 16183 (2003), Abstract; Full Text
Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia: M. Sata, et al.; J. Immunol. 166, 6964 (2001), Abstract;
Vascular endothelial cells and smooth muscle cells differ in expression of Fas and Fas ligand and in sensitivity to Fas ligand-induced cell death: implications for vascular disease and therapy: M. Sata, et al.; Arterioscler. Thromb. Vasc. Biol. 20, 309 (2000), Abstract;
Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis: Y. Refaeli, et al.; Immunity 8, 615 (1998), Abstract;
The regulation of CD95 ligand expression and function in CTL: J.H. Li, et al.; J. Immunol. 161, 3943 (1998), Abstract;
Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?: P. Saas, et al.; J. Clin. Invest. 99, 1173 (1997), Abstract; Full Text
Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover: L.E. French, et al.; J. Cell. Biol. 133, 335 (1996), Abstract;
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996), Abstract;
Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand: T. Renno, et al.; J. Exp. Med. 183, 431 (1996), Abstract;
Characterization of the non-functional Fas ligand of gld mice: M. Hahne, et al.; Int. Immunol. 7, 1381 (1995), Abstract;

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