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LIMK2 monoclonal antibody (10E5-19)

 
ALX-803-344-C100 100 µg 250.00 USD
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Product Specification

Alternative Name:LIM domain kinase 2
 
Clone:10E5-19
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant PDZ domain of LIMK2.
 
UniProt ID:P53671
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human, Mouse
Monkey
 
Specificity:Recognizes full length endogenous LIMK2.
 
Crossreactivity:Does not cross-react with LIMK1.
 
Applications:ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (20ng/ml) , Immunohistochemistry (paraffin sections 10µg/ml) , Immunocytochemistry (10µg/ml) , Western Blot (200ng/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The LIM kinases 1 and 2 (LIMK1 and LIMK2) are activated downstream of the Rho-ROCK, Rac-PAK, and Cdc42-MRCK pathways, thereby acting as network nodes in the signaling pathways that regulate the actin cytoskeleton. LIMK is required for matrix remodeling activities for path generation by leading cells in collective invasion.
 

Product Literature References

LIM kinase 2 is widely expressed in all tissues: K. Acevedo, et al.; J. Histochem. Cytochem. 54, 487 (2006), Abstract;

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