DMPO, mAb (1)



ALX-803-340-C100	100 µg	330.00 USD

Oxidative stress resulting in the formation of biological radicals has been implicated in a number of human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of radical formation. Until now the only general technique that allowed for the detection of these highly reactive species was electron spin resonance (ESR) using spin traps. One of the most popular of these spin traps is 5,5-dimethyl-1-pyrroline N-oxide (DMPO).

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Product Specification

Alternative Name:	5,5-dimethyl-1-pyrroline-N-oxide

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Formulation:	Liquid. In PBS, pH 7.4, containing 10% glycerol and 0.02% sodium azide.

Clone:	1

Isotype:	Mouse IgG

Immunogen:	DMPO-octanoic acid conjugated to ovalbumin.

Source/Host:	Hybridoma tissue culture supernatant.

Specificity:	Recognizes DMPO (Prod. No. ALX-430-090), DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA. Does not cross-react with ovalbumin.

Application:	Immunoprecipitation Western Blot (1-10ug/ml) ELISA (1-10ug/ml) Optimal conditions must be determined individually for each application.

Long Term Storage:	+4°C

Handling:	Do not freeze. Dilute only prior to immediate use.

Figure: Western Blot analysis using DMPO, mAb (1) (Prod. No. ALX-803-340).
Method:Samples are 2.5 µg each from reactions containing:
1= 10 µM Mb only
2= 10 µM Mb + 100 mM DMPO
3= 10 µM Mb + 100 µM H2O2 + 100 mM DMPO
4= 10 µM Mb + 10 µM H2O2 + 10 mM DMPO
Western conditions are:
-either 0.1 or 1.0 µg/ml anti-DMPO IgG for 1 hr at rt in 1XPBS/0.5% Tween
-wash 4X in 1XPBS/0.5% Tween
-1:10,000 anti-mouse IR800 for 1 hr at rt in 1XPBS/0.5% Tween
-wash 2X in 1XPBS/0.5% Tween, wash 2X in PBS
-rinse in H2O
-Scan on Licor Odyssey

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Product Literature References

Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice: S. Chatterjee, et al.; Free Radic. Biol. Med. 46, 454 (2009), Abstract;