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B4GALT1 (human) monoclonal antibody (GT2/36/118)

 
ALX-803-339-C050 50 µg 226.00 USD
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Product Specification

Alternative Name:β-1,4-Galactosyltransferase 1, GGTB2
 
Clone:GT2/36/118
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant human B4GALT1
 
UniProt ID:P15291
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with mouse B4GALT1.
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:500)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. Contains 150µg/ml BSA.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:β-1,4-Galactosyltransferase 1 (B4GALT1) is one of seven B4GALT genes. Human B4GALT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa 78 to 398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains α-lactalbumin. The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.Defects in B4GALT1 are the cause of multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency.
 
803-339

Figure: Immunocytochemistry using B4GALT1, mAb (GT2/36/118) (Prod. No. ALX-803-339). The specificity of the monoclonal antibody was confirmed by immunofluorescence staining of the Golgi apparatus in HeLa cells. Method: B4GALT1, mAb (GT2/36/118) was diluted 1:500 and applied to saponin-permeabilized HeLa cells (after fixation in 3% paraformadehyde). A goat-anti-mouse Alexa488 antibody was used as secondary antibody. DAPI was used to stain the nuclei. The Golgi apparatus shows as a typical perinuclear structure.

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803-339

Product Literature References

Golgi function and dysfunction in the first COG4-deficient CDG type II patient: E. Reynders, et al.; Hum. Mol. Gen. 18, 3244 (2009), Abstract;
Clathrin-dependent Association of CVAK104 with Endosomes and the Trans-Golgi Network: M. Düwel & E.J. Ungewickell; Mol. Biol. Cell 17, 4513 (2006), Abstract;
Golgi Inheritance in Mammalian Cells Is Mediated through Endoplasmic Reticulum Export Activities: N. Altan-Bonnet; Mol. Biol. Cell 17, 990 (2006), Abstract;
Tumor Necrosis Factor-α Up-regulates the Expression of β1,4-Galactosyltransferase I in Primary Human Endothelial Cells by mRNA Stabilization: J.J. Garcia-Vallejo, et al.; J. Biol. Chem. 280, 12676 (2005), Abstract;
The Palmitoyltransferase of the Cation-dependent Mannose 6-Phosphate Receptor Cycles between the Plasma Membrane and Endosomes: J. Stöckli & J. Rohrer; Mol. Biol. Cell 15, 2617 (2004), Abstract;
SseG, a virulence protein that targets Salmonella to the Golgi network: S.P. Salcedo & D.W. Holden; EMBO J. 22, 5003 (2003), Abstract;
Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the trans-Golgi Network: J. Rohrer & R. Kornfeld; Mol. Biol. Cell 12, 1623 (2001), Abstract;
Dynamics of Transitional Endoplasmic Reticulum Sites in Vertebrate Cells: A.T. Hammond & B.S. Glick; Mol. Biol. Cell 11, 3013 (2000), Abstract;
Endoplasmic reticulum-to-cytosol transport of free polymannose oligosaccharides in permeabilized HepG2 cells: S.E.H. Moore, et al.; EMBO J. 14, 6034 (1995), Abstract;
Kin recognition between medial Golgi enzymes in HeLa cells: T. Nilsson, et al.; EMBO J. 13, 562 (1994), Abstract;
The TGN38 Glycoprotein Contains Two Non-Overlapping Signals That Mediate Localization to the Trans-Golgi Network: S. Ponnambalam, et al.; J. Cell Biol. 125, 253 (1994), Abstract;
Giantin, a Novel Conserved Golgi Membrane Protein Containing a Cytoplasmic Domain of at Least 350 kDa: A.D. Linstedt & HP. Hauri; Mol. Biol. Cell 4, 679 (1993), Abstract;
Monoclonal antibodies to soluble human milk galactosyltransferase (lactose synthase A protein): E.G. Berger, et al.; Carbohydr. Res. 149, 23 (1986), Abstract;

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