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VRK1 (human) (1F6) monoclonal antibody

 
ALX-803-321-C100 100 µg 280.00 USD
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Product Specification

Alternative Name:Serine/threonine protein kinase VRK1, Vaccinia-related kinase 1
 
Clone:1F6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human VRK1 (vaccinia-related kinase 1).
 
UniProt ID:Q99986
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with mouse or rat VRK1.
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:500)
Western Blot (1:1,000-1:10,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Vaccinia-related kinase 1 (VRK1) contributes to p53 stabilization by partly interfering with its MDM2-mediated degradation.
 
803-321
Figure: Western Blot of endogenous Vaccinia-related Kinase 1 (VRK1) (human) by the MAb to VRK1(1F6) (Prod. No. ALX-803-321). Method: Total cell extracts (10µg) of human embryo kidney 293 (HEK293) (lane 1) and HeLa (lane 2) cell lines were run on a SDS-gel, and revealed with MAb to VRK1(1F6) (1:1’000) for 1 hour at room temperature followed by incubation with HRP-coupled goat-anti mouse antibody (1:4’000). The arrow indicates endogenous VRK1. 
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803-321

Product Literature References

Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.: I. López- Sánchez, et al.; Mol. Cell Biol. 29, 1189 (2009), Abstract;
A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection: N.J. MacLaine; J. Biol. Chem. 283, 28563 (2008), Abstract;
Human VRK1 Is an Early Response Gene and Its Loss Causes a Block in Cell Cycle Progression: A. Valbuena, et al.; PLOS ONE 3, e1642 (2008), Abstract;
Proteomic identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (Vaccinia-Related Kinase) activities: M. Sanz-García; Mol. Cell. Proteomics 7, 2199 (2008), Abstract;
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations: A. Valbuena, et al.; Arch. Biochem. Biophys. 465, 219 (2007), Abstract;
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma: C.R. Santos, et al.; Mol. Cancer Res. 4, 177 (2006), Abstract;
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1: F.M. Vega, et al.; Mol. Cell. Biol. 24, 10366 (2004), Abstract;

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