United States
RUNX3, mAb (R3-1E10)
| ALX-803-320-C100 | 100 µg | 350.00 USD |  |
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Product Specification
| Alternative Name: | Acute myeloid leukemia 2 protein, Runt-related transcription factor 3, CBF-α3, AML-2, Core-binding factor α3 subunit |
| Clone: | R3-1E10 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300). |
| UniProt ID: | Q13761 |
| Application: | Immunohistochemistry (paraffin sections (2µg/ml)) Western Blot (0.2µg/ml) |
| Specificity: | Recognizes human and mouse RUNX3. |
| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
| Handling: | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Miscellaneous/General: | RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells. |
Figure 1: Western blot analysis using MAb to RUNX3 (R3-1E10) (Prod. No. ALX-803-320). Method: 0.2µg/ml of MAb to RUNX3 (R3-1E10) were applied to the membrane on which recombinant human (lanes 1-3) or mouse (lanes 4-6) RUNX1, RUNX2 or RUNX3 expressed in COS7 cells were loaded (see [1]).
Figure 2: Immunohistochemistry using MAb to RUNX3 (R3-1E10) (Prod. No. ALX-803-320).Method: 2µg/ml of MAb to RUNX3 (R3-1E10) were applied to normal human gastric samples (paraffin sections) which were retrieved by autoclave at 105°C for 15 min. in Dako solution (see [1]).
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Product Literature References
The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis: T. Yano, et al.; Mol. Cell. Biol. 26, 4474 (2006), Abstract; Full Text
General Literature References
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer: Q.C. Lau, et al.; Cancer Res. 66, 6512 (2006), Abstract; Full Text
Tumor suppressor activity of RUNX3: S.C. Bae & J.K. Choi; Oncogene 23, 4336 (2004), Abstract;
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