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RUNX3 (human) monoclonal antibody (R3-6E9)

 
ALX-803-318-C100 100 µg 363.00 USD
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Product Specification

Alternative Name:Core-binding factor α3 subunit, AML-2, Acute myeloid leukemia 2 protein, CBF-α3, Runt-related transcription factor 3
 
Clone:R3-6E9
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
 
UniProt ID:Q13761
 
Species reactivity:Human
 
Crossreactivity:Weakly cross-reacts with mouse RUNX3.
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 2µg/ml)
Western Blot (0.2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogenesis. Of the three known RUNX family members, the ubiquitously expressed RUNX3 is involved in neurogenesis, T cell differentiation, tumorigenesis and is a component of the TGF-β induced tumor suppressor pathway. In gastric cancer cells, RUNX3 induces cell cycle arrest and apoptosis through upregulation of Bim, a proapoptotic BH3-only protein. In addition, RUNX3 is frequently inactivated in gastric cancer cells.
 
803-318
Figure 1: Western blot analysis using MAb to RUNX3 (human) (R3-6E9) (Prod. No. ALX-803-318). Method: 0.2µg/ml of MAb to RUNX3 (human) (R3-6E9) were applied to the membrane on which recombinant human (lanes 1-3) or mouse (lanes 4-6) RUNX1, RUNX2 or RUNX3 expressed in COS7 cells were loaded (see [1]).
803-318 ihc
Figure 2: Immunohistochemistry using MAb to RUNX3 (human) (R3-6E9) (Prod. No. ALX-803-318). Method: 2µg/ml of MAb to RUNX3 (human) (R3-6E9) were applied to normal human gastic samples (paraffin sections) which were retrieved by autoclave at 105°C for 15 min. in Dako solution (see [1]).
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803-318 803-318 ihc

Product Literature References

Targets of genome copy number reduction in primary breast cancers identified by integrative genomics: W. Chen, et al.; Genes Chromosomes Cancer 46, 288 (2007), Abstract;
RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer: Q.C. Lau, et al.; Cancer Res. 66, 6512 (2006), Abstract; Full Text
RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization: K. Ito, et al.; Cancer Res. 65, 7743 (2005), Abstract; Full Text

General Literature References

The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis: T. Yano, et al.; Mol. Cell. Biol. 26, 4474 (2006), Abstract; Full Text
Tumor suppressor activity of RUNX3: S.C. Bae & J.K. Choi; Oncogene 23, 4336 (2004), Abstract;

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