Glutamate decarboxylase (65kDa isoform) (human), mAb (61/8F9)



ALX-803-055-C100	100 µg	309.00 USD

Product Specification

Alternative Name:	GAD65, GAD2

Concentration:	0.5mg/ml

Purity Detail:	Ammonium sulphate precipitation.

Formulation:	Liquid. In PBS. pH 7.4, containing 0.9mg/ml sodium azide.

Clone:	61/8F9

Isotype:	Mouse IgG1

Immunogen:	Recombinant human GAD65 (glutamate decarboxylase (65kDa isoform).

Specificity:	Recognizes an epitope (aa 4-17) of human GAD65. Does not cross-react with GAD67.

Application:	Immunohistochemistry (frozen sections) Immunoprecipitation Radioimmunoassay Western Blot

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year when stored at +4°C.

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Striking binding to islets of cryosections of human, monkey, pig and rat pancreas but not on mouse pancreas. Not detectable on Bouin-fixed pancreatic tissues. Immunostaining of the Purkinje cell layer of the NOD mouse cerebellum. No binding to viable rat islet cells.

Product Literature References

A monoclonal antibody-based characterization of autoantibodies against glutamic acid decarboxylase in adults with latent autoimmune diabetes: B. Ziegler, et al.; Autoimmunity 28, 61 (1998), Abstract;

Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions: P. Augstein, et al.; Pancreas 15, 139 (1997), Abstract;

Comparison of the islet cell antibody pattern of monoclonal glutamic acid decarboxylase antibodies recognizing linear and conformational epitopes: P. Augstein, et al.; Acta Histochem. 98, 229 (1996), Abstract;

Monoclonal antibodies specific to the glutamic acid decarboxylase 65 kDa isoform derived from a non-obese diabetic (NOD) mouse: B. Ziegler, et al.; Diabetes Res. 25, 47 (1994), Abstract;