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Glutamate decarboxylase (65kDa isoform) (human) monoclonal antibody (83/2E6)

 
ALX-803-054-C100 100 µg 310.00 USD
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Product Specification

Alternative Name:GAD65, GAD2
 
Clone:83/2E6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human GAD65 (glutamate decarboxylase (65kDa isoform)).
 
UniProt ID:Q05329
 
Species reactivity:Human, Rat
 
Specificity:Recognizes an epitope (aa 221-442) of human GAD65. Binds to human and rat pancreas tissue but not mouse pancreas tissue. Not detectable on Bouin-fixed pancreatic tissues. No binding to viable rat islet cells.
 
Crossreactivity:Does not cross-react with GAD67.
 
Applications:ICC, IHC (FS), IP
Radioimmunoassay
 
Application Notes:GAD-binding of this antibody is competitively inhibited by human GAD-antibodies.
 
Purity Detail:Ammonium sulphate precipitation.
 
Formulation:Liquid. In PBS. pH 7.4, containing 0.9mg/ml sodium azide.
 
Use/Stability:Stable for at least 1 year when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

A monoclonal antibody-based characterization of autoantibodies against glutamic acid decarboxylase in adults with latent autoimmune diabetes: B. Ziegler, et al.; Autoimmunity 28, 61 (1998), Abstract;
Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions: P. Augstein, et al.; Pancreas 15, 139 (1997), Abstract;
Comparison of the islet cell antibody pattern of monoclonal glutamic acid decarboxylase antibodies recognizing linear and conformational epitopes: P. Augstein, et al.; Acta Histochem. 98, 229 (1996), Abstract;
Monoclonal antibodies specific to the glutamic acid decarboxylase 65 kDa isoform derived from a non-obese diabetic (NOD) mouse: B. Ziegler, et al.; Diabetes Res. 25, 47 (1994), Abstract;

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