Glutamate decarboxylase (65kDa isoform) (human), mAb (83/2E6)



ALX-803-054-C100	100 µg	309.00 USD

Product Specification

Alternative Name:	GAD65, GAD2

Concentration:	0.5mg/ml

Purity Detail:	Ammonium sulphate precipitation.

Formulation:	Liquid. In PBS. pH 7.4, containing 0.9mg/ml sodium azide.

Clone:	83/2E6

Isotype:	Mouse IgG1

Immunogen:	Recombinant human GAD65 (glutamate decarboxylase (65kDa isoform)).

Specificity:	Recognizes an epitope (aa 221-442) of human GAD65. Binds to human and rat pancreas tissue but not mouse pancreas tissue. Not detectable on Bouin-fixed pancreatic tissues. No binding to viable rat islet cells. Does not cross-react with GAD67. GAD-binding of this antibody is competitively inhibited by human GAD-antibodies.

Application:	Immunocytochemistry Immunoprecipitation Radioimmunoassay

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year when stored at +4°C.

Handling:	Avoid freeze/thaw cycles.

Product Literature References

A monoclonal antibody-based characterization of autoantibodies against glutamic acid decarboxylase in adults with latent autoimmune diabetes: B. Ziegler, et al.; Autoimmunity 28, 61 (1998), Abstract;

Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions: P. Augstein, et al.; Pancreas 15, 139 (1997), Abstract;

Comparison of the islet cell antibody pattern of monoclonal glutamic acid decarboxylase antibodies recognizing linear and conformational epitopes: P. Augstein, et al.; Acta Histochem. 98, 229 (1996), Abstract;

Monoclonal antibodies specific to the glutamic acid decarboxylase 65 kDa isoform derived from a non-obese diabetic (NOD) mouse: B. Ziegler, et al.; Diabetes Res. 25, 47 (1994), Abstract;