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Viable rat beta cells and RIN cells monoclonal antibody (K14D10)

 
ALX-803-052-C100 100 µg 562.00 USD
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Product Specification

Clone:K14D10
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Pig proinsulin.
 
Species reactivity:Rat
 
Specificity:Recognizes surface structure of viable isolated rat beta cells. Does not recognize mouse islet beta cells.
 
Crossreactivity:Cross-reactivity with human pancreatic beta cells was not detected.
 
Applications:Flow Cytometry, ICC, IHC (FS)
Cell sorting
 
Application Notes:Not recommended for WB and IP.
 
Purity Detail:Ammonium sulphate precipitation.
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.9mg/ml sodium azide.
 
Use/Stability:Stable for at least 1 year when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Immunomagnetic purification of beta cells from rat islets of Langerhans: V. Hadjivassiliou, et al.; Diabetologia 43, 1170 (2000), Abstract;
Recognition of islet-directed peripheral blood lymphocytes in vitro before rejection of allogeneic grafted islets: B. Kuttler, et al.; Transplantation 69, 1987 (2000), Abstract;
Co-culture of pancreatic islets and allogeneic lymphocytes: alterations of responder and stimulator cells: B. Kuttler, et al.; Transplantation 64, 480 (1997), Abstract;
Monoclonal antibody-mediated cytotoxicity against rat beta cells detected in vitro does not cause beta-cell destruction in vivo: B. Ziegler, et al.; Diabetologia 35, 608 (1992), Abstract;
Influence of target cell preparation on binding of monoclonal islet cell reactive antibodies (mc-ICRA) in cellular enzyme-linked immunosorbent assay (CELISA): M. Schlosser, et al.; J. Immunol. Methods 140, 101 (1991), Abstract;

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