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Estrogen receptor α monoclonal antibody (33)

 
ALX-803-004-C050 50 µg 552.00 USD
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Product Specification

Alternative Name:ERα
 
Clone:33
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α). This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
 
UniProt ID:P03372
 
Species reactivity:Human, Mouse, Rat
Bovine, Monkey
 
Specificity:Recognizes both the steroid occupied and unoccupied forms of ERα.
 
Crossreactivity:Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ.
 
Applications:Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Immunohistochemistry (frozen sections and paraffin sections, 5µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining.
Detects a band of ~67kDa by Western blot.
Does not inhibit binding of ER to nuclei and DNA.
 
Formulation:Liquid. In PBS containing 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Steroid receptors are a large superfamily of ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Estrogen receptor (ER) has been classified into two distinct isoforms, α and β. ERα is known to interact in a ligand dependent and independent manner with several known coactivators including Receptor-Interacting Protein 140 (RIP140/ERAP140), ERAP160, and Steroid Receptor Coactivator-1 (SRC-1) to enhance transcriptional activity of target genes.
 
803-004
Figure: Western blot of recombinant ERa using MAb to Estrogen Receptor α (33) (Prod. No. ALX-803-004).
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803-004

Product Literature References

Histochemical and electrophysiological evidence for estrogen receptors on cultured astrocytes: colocalization with cholinergic receptors: E. Hosli, et al.; Int. J. Dev. Neurosci. 18, 101 (2000), Abstract;
Measurement of estrogen receptors in intact cells by flow cytometry: S. Cao, et al.; Cytometry 41, 109 (2000), Abstract;
Cellular localization of estrogen receptors on neurones in various regions of cultured rat CNS: coexistence with cholinergic and galanin receptors: E. Hosli & L. Hosli; Int. J. Dev. Neurosci. 17, 317 (1999), Abstract;
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor: A. Traish, et al.; Steroids 55, 196 (1990), Abstract;

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