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[pThr286]Ca2+/calmodulin-dependent protein kinase II (rat) (α subunit) monoclonal antibody (22B1)

 
ALX-802-009-R100 100 µl 420.00 USD
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Replaces Prod. #: BML-SA234

Product Specification

Alternative Name:CaM kinase II α subunit, CAMK2 α subunit
 
Clone:22B1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 281-294 (M281HRQET(PSO3)VDCLKKFN294) of the α-subunit of mouse and rat CaM kinase II (Ca2+/Calmodulin-dependent Protein Kinase II) thiophosphorylated at (Thr286).
 
UniProt ID:P11798 (mouse), P11275 (rat)
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Recognizes the phosphorylated α-subunit of CaM kinase II. This antibody does not detect the non-phosphorylated α-subunit of rat CaM kinase II nor the ~60kDa β-subunit in either phosphorylation state.
 
Applications:ELISA, Flow Cytometry, ICC, IF, IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (FACS, 1μg 106 cells)
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II: C.M. Atkins, et al.; J. Neurosci. 24, 5193 (2004), Abstract; Full Text
Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development: L.M. Esteban, et al.; Mol. Cell. Biol. 21, 1444 (2001), Abstract; Full Text
Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies: B.L. Patton, et al.; Mol. Biol. Cell 4, 159 (1993), Abstract;

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