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Nicotinic acetylcholine receptor monoclonal antibody (88B)

 
ALX-802-002-R100 100 µl 399.00 USD
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Product Specification

Alternative Name:nAChR
 
Clone:88B
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Purified Torpedo californica AChR (acetylcholine receptor).
 
Species reactivity:Mouse, Rat
Chicken, Fish, Frog, Torpedo californica
 
Specificity:Recognizes the intracellular domain of skeletal muscle nAChR γ and δ subunits in Torpedo californica and the δ subunit in mouse, rat, chicken, frog and fish.
 
Crossreactivity:Does not cross-react with the α1 and β1 subunits in muscle or the neuronal nAChRs.
 
Applications:IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1:1,000)
Western Blot (1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~60kDa (γ subunit ) and ~65kDa (δ subunit) by Western blot. Under non-reducing conditions detects a band of ~130kDa (dimer of δ subunit).
 
Formulation:Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Structure of nicotinic acetylcholine receptors: A. Karlin; Curr. Opinion Neurobiol. 3, 299 (1993), Abstract;
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor: S.C. Froehner, et al.; J. Biol. Chem. 258, 7112 (1983), Abstract; Full Text

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