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α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R), mAb (M86)

 
ALX-801-090-1 1 Vial 320.00 USD
 
Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary).

Product Specification

Formulation:Liquid. Tissue culture supernatant.
 
Clone:M86
 
Isotype:Mouse IgM
 
Immunogen:Rabbit red blood cell membrane.
 
Source/Host:Hybridoma tissue culture.
 
Specificity:Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids. Does not cross-react with β-Gal glycoproteins or BSA.
 
Application:ELISA
Flow Cytometry
Immunohistochemistry (paraffin sections)
Western Blot
Functional Application (can be used for cytotoxicity assays for α-Gal specific pathways)
Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody.
Appropriate antibody dilution may vary from lot to lot.  An initial dilution of 1:5  is recommended, if not otherwise indicated.
Optimal conditions must be determined individually for each application.
 
Shipping:SHIPPED ON BLUE ICE
 
Long Term Storage:-20°C
 
Use/Stability:

Stable for at least 3 years when stored at -20°C.

 
Handling:After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
 

Product Literature References

A sensitive assay for measuring alpha-Gal epitope expression on cells by a monoclonal anti-Gal antibody: U. Galili, et al.; Transplantation 65, 1129 (1998), Abstract;
alpha-Gal Oligosaccharides: Chemistry and Potential Biomedical Application: A. Janczuk, et al.; Current Med. Chem. 6, 155 (1999), Abstract;
Regulation of natural killer cell-mediated swine endothelial cell lysis through genetic remodeling of a glycoantigen: S. Miyagawa, et al.; J. Biochem. 126, 1067 (1999), Abstract;
Differential expression of alpha-Gal epitopes on pig and mouse organs: M. Tanemura & U. Galili; Transplant. Proc. 32, 843 (2000), Abstract;
Differential immune responses to alpha-gal epitopes on xenografts and allografts: implications for accommodation in xenotransplantation: M. Tanemura, et al.; J. Clin. Invest. 105, 301 (2000), Abstract;
Mechanism of delayed rejection in transgenic pig-to-primate cardiac xenotransplantation: R.H. Chen, et al.; J. Surg. Res. 90, 119 (2000), Abstract;
Xenotransplantation: in vitro analysis of synthetic alpha-galactosyl inhibitors of human anti-Galalpha1-->3Gal IgM and IgG antibodies: R. Rieben, et al.; Glycobiology 10, 141 (2000), Abstract;
Remodeling of the Major Pig Xenoantigen by N-Actetylglucosaminyltransferase III in Transgenic Pig: S. Miyagawa, et al.; J. Biol. Chem. 276, 39310 (2001), Abstract; Full Text
Synthesis of α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) on human tumor cells by recombinant α1,3galactosyltransferase produced in Pichia pastoris: Z.C. Chen, et al.; Glycobiology 11, 577 (2001), Abstract; Full Text
The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) in xenotransplantation: U. Galili; Biochemie 83, 557 (2001), Abstract;
Differential expression of Galalpha1,3Gal epitopes on fetal and adult porcine hematopoietic cells: S. Gojo, et al.; Xenotransplantation 9, 297 (2002), Abstract; Full Text
Expression of alpha-gal epitopes on HeLa cells transduced wtih adenovirus containing alpha-1,3galactosyltransferase cDNA: L. Deriy, et al.; Glycobiology 12, 135 (2002), Abstract;
Lack of Galactose-alpha-1,3-Galactose Expression on Porcine Endothelial Cells Prevents Complement-Induced Lysis but Not Direct Xenogeneic NK Cytotoxicity: B.C. Baumann, et al.; J. Immunol. 172, 6460 (2004), Abstract;

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