Product Details
Alternative Name: | Multidrug resistance-associated protein 4, ABCC4 |
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Clone: | M4I-10 |
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Host: | Rat |
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Isotype: | IgG2a |
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Immunogen: | Recombinant human MRP4 (multidrug resistance-associated protein 4) (aa 372-431). |
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UniProt ID: | O15439 |
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Species reactivity: | Human, Mouse
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Applications: | ICC, IHC (FS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations. Immunohistochemistry (frozen sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with biotin-labelled rabbit anti-rat IgG and HRP-labelled streptavidin. Western Blot: Use 1:20-1:50 dilution and HRP-anti-rat-IgG. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunocytochemistry: cytospin preparations |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Alteration of human hepatic drug transporter activity and expression by cigarette smoke condensate: K. Sayyed, et al.; Toxicology
363, 58 (2016),
Application(s): Cell culture; Effects on ABC transporter activities,
Abstract;
N-linked glycans do not affect plasma membrane localization of multidrug resistance protein 4 (MRP4) but selectively alter its prostaglandin E2 transport activity: M.F. Miah, et al.; Biochem. Biophys. Res. Commun.
469, 954 (2016),
Application(s): Immunoblotting,
Abstract;
Down-regulation of Na+/H+ Exchanger Regulatory Factor 1 Increases Expression and Function of Multidrug Resistance Protein 4: M.T. Hoque & S.P. Cole; Cancer Res.
68, 4802 (2008),
Abstract;
Full Text
Mrp4 confers resistance to topotecan and protects the brain from chemotherapy: M. Leggas, et al.; Mol. Cell. Biol.
24, 7612 (2004),
Abstract;