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MRP5 (human) monoclonal antibody (M5I-1)

 
ALX-801-021-C250 250 µg 735.00 USD
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Product Specification

Alternative Name:Multidrug resistance-associated protein 5, ABCC5
 
Clone:M5I-1
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human MRP5 (multidrug resistance-associated protein 5) (aa 82-168).
 
UniProt ID:O15440
 
Species reactivity:Human
 
Specificity:Recognizes an internal epitope of human MRP5.
 
Crossreactivity:Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP3.
 
Applications:Flow Cytometry, ICC, IHC (FS), WB
 
Recommended Dilutions/Conditions:Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.
Immunohistochemistry (frozen sections, weak): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase.
Western Blot: Use 1:20-1:50 dilution and anti-rat-HRP.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
 
Application Notes:Flow Cytometry: weak, cell permeabilization required
Immunocytochemistry: cytospin preparations
 
Formulation:Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000), Abstract; Full Text

General Literature References

Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res. 57, 3537 (1997), Abstract;

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