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MRP2 monoclonal antibody (M2III-6)

 
ALX-801-016-C250 250 µg 830.00 USD
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Product Specification

Alternative Name:Multidrug resistance-associated protein 2, cMOAT, ABCC2
 
Clone:M2III-6
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant C-terminal rat MRP2 (multidrug resistance-associated protein-2; cMOAT) (aa 1339-1541).
 
UniProt ID:Q63120
 
Species reactivity:Human, Rat
 
Specificity:Recognizes an internal epitope of MRP2.
 
Crossreactivity:Does not cross-react with human P-glycoprotein, MDR3, MRP1, MRP3 or MRP5.
 
Applications:ICC, IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations., Immunohistochemistry (frozen sections) (1:20): Can be use on acetone fixed frozen sections. Can be followed by incubation with rabbit anti-mouse antibody and a monoclonal mouse APAAP complex.
Immunohistochemistry (paraffin sections) (1:20, weak): Can be used on formaldehyde-fixed paraffin-embedded tissues and tumors, after pretreatment with 0.01M citric acid (pH 6.0) in distilled water at 100°C for 5 min 3 times. After incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-mouse antibody and streptavidin conjugated to horseradish peroxidase.
Western Blot: Use 1:20-1:50 and anti-mouse HRP.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Alteration of human hepatic drug transporter activity and expression by cigarette smoke condensate: K. Sayyed, et al.; Toxicology 363, 58 (2016), Application(s): Cell culture; Effects on ABC transporter activities, Abstract;
Analysis of the Bile Salt Export Pump (ABCB11) Interactome Employing Complementary Approaches: S. Przybylla, et al.; PLoS One 11, e0159778 (2016), Application(s): Immunofluorescence staining of liver cryosections and differentiated HepaRG cells, Abstract; Full Text
Exon-skipping and mRNA decay in human liver tissue: molecular consequences of pathogenic bile salt export pump mutations: C. Droge, et al.; Sci. Rep. 6, 24827 (2016), Application(s): Immunofluorescence of liver tissue, Abstract;
Mitogen-activated protein kinases are involved in hepatocanalicular dysfunction and cholestasis induced by oxidative stress: F.D. Toledo, et al.; Arch. Toxicol. (2016), Abstract;
The antitripanocide benznidazole promotes adaptive response to oxidative injury: Involvement of the nuclear factor-erythroid 2-related factor-2 (Nrf2) and multidrug resistance associated protein 2 (MRP2): J.P. Rigalli, et al.; Toxicol. Appl. Pharmacol. 304, 90 (2016), Application(s): Western blot, Abstract;
Anti-apoptotic effect of modified Chunsimyeolda-tang, a traditional Korean herbal formula, on MPTP-induced neuronal cell death in a Parkinson's disease mouse model: H. Li, et al.; J. Ethnopharmacol. 176, 336 (2015), Application(s): Western blot, Abstract;
Coordinated induction of GST and MRP2 by cAMP in Caco-2 cells: Role of protein kinase A signaling pathway and toxicological relevance: M.R. Arana, et al.; Toxicol. Appl. Pharmacol. 287, 178 (2015), Application(s): Cell culture , Abstract;
Evaluation of transport of common antiepileptic drugs by human multidrug resistance-associated proteins (MRP1, 2 and 5) that are overexpressed in pharmacoresistant epilepsy: C. Luna-Tortos, et al.; Neuropharmacology 58, 1019 (2010), Abstract;
Multiple pathways for fluoroquinolone secretion by human intestinal epithelial (Caco-2) cells: S. Lowes, et al.; Br. J. Pharmacol. 135, 1263 (2002), Abstract; Full Text
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res. 60, 5269 (2000), Abstract; Full Text
Antifolate resistance mediated by the multidrug resistance proteins MRP1 and MRP2: J.H. Hooijberg, et al.; Cancer Res. 59, 2532 (1999), Abstract; Full Text
Drug export activity of the human canalicular multispecific organic anion transporter in polarized kidney MDCK cells expressing cMOAT (MRP2) cDNA: R. Evers, et al.; J. Clin. Invest. 101, 1310 (1998), Abstract; Full Text
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science 271, 1126 (1996), Abstract;

General Literature References

Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines  : M. Kool, et al.; Cancer Res. 57, 3537 (1997), Abstract;

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