Product Details
| Alternative Name: | Multidrug resistance-associated protein 1, ABCC1 |
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| Clone: | QCRL-1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Membranes prepared from the human small cell lung cancer cell line H69AR. |
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| UniProt ID: | P33527 |
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| Species reactivity: | Human
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| Specificity: | Recognizes the linear internal epitope (S918SYSGDI924) of human MRP1 (multidrug resistance-associated protein 1), overexpressed in various human non-P-glycoprotein MDR tumor cell lines. |
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| Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP5, or mouse MRP gene products. |
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| Applications: | Flow Cytometry, ICC, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:20)
Immunocytochemistry (1:20-1:50)
Western Blot (0.02-0.2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: cell permeabilization required
Immunocytochemistry: cytospin preparations |
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| Formulation: | Liquid. 0.22μm-filtered solution in PBS containing 0.7% BSA and 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Human MRP1-specific MAb QCRL-1 (Prod.No.
ALX-801-010)."
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Product Literature References
Rhodamine 123 binds to multiple sites in the multidrug resistance protein (MRP1): R. Daoud, et al.; Biochemistry
39, 15344 (2000),
Abstract;
The multidrug resistance protein is photoaffinity labeled by a quinoline-based drug at multiple sites: R. Daoud, et al.; Biochemistry
39, 6094 (2000),
Abstract;
ATPase activity of purified and reconstituted multidrug resistance protein MRP1 from drug-selected H69AR cells: Q. Mao, et al.; Biochim. Biophys. Acta
1461, 69 (1999),
Abstract;
Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier: V.V. Rao, et al.; PNAS
96, 3900 (1999),
Abstract;
Full Text
Expression of the multidrug resistance protein (MRP1) in breast cancer: M. Filipits, et al.; Anticancer Res.
19, 5043 (1999),
Abstract;
MRP expression in acute myeloid leukemia. An update: M. Filipits, et al.; Adv. Exp. Med. Biol.
457, 141 (1999),
Abstract;
Epitope mapping of monoclonal antibodies specific for the 190-kDa multidrug resistance protein (MRP): D.R. Hipfner, et al.; Br. J. Cancer
78, 1134 (1998),
Abstract;
Immunohistochemical detection of multidrug resistance protein in human lung cancer and normal lung: S.R. Wright, et al.; Clin. Cancer Res.
4, 2279 (1998),
Abstract;
Location of a protease-hypersensitive region in the multidrug resistance protein (MRP) by mapping of the epitope of MRP-specific monoclonal antibody QCRL-1: D.R. Hipfner, et al.; Cancer Res.
56, 3307 (1996),
Abstract;
MRP and MDR1 gene expression in primary breast carcinomas: M. Filipits, et al.; Clin. Cancer Res.
2, 1231 (1996),
Abstract;
Characterization of the M(r) 190,000 multidrug resistance protein (MRP) in drug-selected and transfected human tumor cell: K.C. Almquist, et al.; Cancer Res.
55, 102 (1995),
Abstract;
Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies: D.R. Hipfner, et al.; Cancer Res.
54, 5788 (1994),
Abstract;
