Product Details
| Alternative Name: | Multidrug resistance-associated protein 1, ABCC1 |
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| Clone: | MRPr1 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human MRP1 (multidrug resistance-associated protein 1) (aa 192-360). |
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| UniProt ID: | P33527 |
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| Species reactivity: | Human, Mouse
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| Specificity: | Recognizes the internal epitope (G238SDLWSLNKE247) of MRP1, overexpressed in various human non-P-glycoprotein MDR tumor cell lines. |
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| Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein or human MRP2 (cMOAT) to MRP 5. |
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| Applications: | Flow Cytometry, ICC, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Suggested starting dilution is 1:20
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Flow Cytometry: cell permeabilization required
Immunocytochemistry: cytospin preparations |
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| Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Epitope Mapping of MAbs MRPr1, QCRL-1 & MRPm6. Topological model of MRPdepicting the approx. locations of the MRPr1, QCRL-1 and MRPm6 epitopes. The model shown is only one of several possible configurations. MSD=membrane spanning domain; NBD=nucleotide binding domain. From R. Hipfner, et al.; Br. J. Cancer 78,1134 (1998).
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Product Literature References
Localization of MRP-1 to the outer mitochondrial membrane by the chaperone protein HSP90β: E. Roundhill, et al.; FASEB J.
30, 1712 (2016),
Abstract;
Characterization of efflux transport proteins of the human choroid plexus papilloma cell line HIBCPP, a functional in vitro model of the blood-cerebrospinal fluid barrier: A. Bernd, et al.; Pharm. Res.
32, 2973 (2015),
Abstract;
Identification of new MRP4 inhibitors from a library of FDA approved drugs using a high-throughput bioluminescence screen: L. Cheung, et al.; Biochem. Pharmacol.
93, 380 (2015),
Application(s): Western blot with HEK293 cells,
Abstract;
Inhibition of Multidrug resistance protein 1 (MRP1) improves chemotherapy drug response in primary and recurrent glioblastoma multiforme: A. Tivnan, et al.; Front. Neurosci.
9, 218 (2015),
Application(s): Western Blot,
Abstract;
Full Text
The effect of maternal obesity on the expression and functionality of placental P-glycoprotein: Implications in the individualized transplacental digoxin treatment for fetal heart failure: C. Wang, et al.; Placenta
36, 1138 (2015),
Application(s): Western blot with human placenta,
Abstract;
Full Text
High-throughput screening identifies Ceefourin 1 and Ceefourin 2 as highly selective inhibitors of multidrug resistance protein 4 (MRP4): L. Cheung, et al.; Biochem. Pharmacol.
91, 97 (2014),
Application(s): Western blot with MCF7 cells,
Abstract;
Effect of multiple cysteine substitutions on the functionality of human multidrug resistance protein 1 expressed in human embryonic kidney 293 cells: identification of residues essential for function: L. Qin, et al.; Drug. Metab. Dispos.
40, 1403 (2012),
Application(s): Immunofluorescence using HEK293 cells,
Abstract;
Inflammatory regulation of ATP binding cassette efflux transporter expression and function in microglia: C.J. Gibson, et al.; J. Pharmacol. Exp. Ther.
343, 650 (2012),
Application(s): Western blot with BV-2 cells,
Abstract;
Full Text
Identification and characterization of functionally important elements in the multidrug resistance protein 1 COOH-terminal region: C.J. Westlake, et al.; J. Biol. Chem.
279, 53571 (2004),
Abstract;
Full Text
Rhodamine 123 binds to multiple sites in the multidrug resistance protein (MRP1): R. Daoud, et al.; Biochemistry
39, 15344 (2000),
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
The multidrug resistance protein 1: a functionally important activation marker for murine Th1 cells: S. Prechtl, et al.; J. Immunol.
164, 754 (2000),
Abstract;
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The multidrug resistance protein is photoaffinity labeled by a quinoline-based drug at multiple sites: R. Daoud, et al.; Biochemistry
39, 6094 (2000),
Abstract;
Bcl-2, bax and bcl-xL expression in human sensitive and resistant leukemia cell lines: V. Nuessler, et al.; Leukemia
13, 1864 (1999),
Abstract;
Choroid plexus epithelial expression of MDR1 P glycoprotein and multidrug resistance-associated protein contribute to the blood-cerebrospinal-fluid drug-permeability barrier: V.V. Rao, et al.; PNAS
96, 3900 (1999),
Abstract;
Full Text
Drug resistance-associated markers P-glycoprotein, multidrug resistance-associated protein 1, multidrug resistance-associated protein 2, and lung resistance protein as prognostic factors in ovarian carcinoma: H.J. Arts, et al.; Clin. Cancer Res.
5, 2798 (1999),
Abstract;
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Increased expression of multidrug resistance related proteins Pgp, MRP1, and LRP/MVP occurs early in colorectal carcinogenesis: G.A. Meijer, et al.; J. Clin. Pathol.
52, 450 (1999),
Abstract;
Reproducible flow cytometric methodology for measuring multidrug resistance in leukaemic blasts: M. Pallis, et al.; Adv. Exp. Med. Biol.
457, 77 (1999),
Abstract;
Epitope mapping of monoclonal antibodies specific for the 190-kDa multidrug resistance protein (MRP): D.R. Hipfner, et al.; Br. J. Cancer
78, 1134 (1998),
Abstract;
Relationship between major vault protein/lung resistance protein, multidrug resistance-associated protein, P-glycoprotein expression, and drug resistance in childhood leukemia: M.L. Den Boer, et al.; Blood
91, 2092 (1998),
Abstract;
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Optimal immunocytochemical and flow cytometric detection of P-gp, MRP and LRP in childhood acute lymphoblastic leukemia: M.L. Den Boer, et al.; Leukemia
11, 1078 (1997),
Abstract;
The prognostic significance of expression of the multidrug resistance-associated protein (MRP) in primary breast cancer: K. Nooter, et al.; Br. J. Cancer
76, 486 (1997),
Abstract;
Expression of the multidrug resistance-associated protein (MRP) gene in primary non-small-cell lung cancer: K. Nooter, et al.; Ann. Oncol.
7, 75 (1996),
Abstract;
Methods to detect P-glycoprotein-associated multidrug resistance in patients' tumors: consensus recommendations: W.T. Beck, et al.; Cancer Res.
56, 3010 (1996),
Abstract;
Multidrug resistance-associated protein expression in clinical gastric carcinoma: K. Endo, et al.; Cancer
77, 1681 (1996),
Abstract;
Tissue distribution of the multidrug resistance protein: M.J. Flens, et al.; Am. J. Pathol.
148, 1237 (1996),
Abstract;
Expression of the multidrug resistance-associated protein (MRP) gene in human cancers: K. Nooter, et al.; Clin. Cancer Res.
1, 1301 (1995),
Abstract;
Membrane transport proteins associated with drug resistance expressed in human melanoma: D. Schadendorf, et al.; Am. J. Pathol.
147, 1545 (1995),
Abstract;
Detection of the M(r) 190,000 multidrug resistance protein, MRP, with monoclonal antibodies: D.R. Hipfner, et al.; Cancer Res.
54, 5788 (1994),
Abstract;
Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies: M.J. Flens, et al.; Cancer Res.
54, 4557 (1994),
Abstract;
The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump: G.J. Zaman, et al.; PNAS
91, 8822 (1994),
Abstract;
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