| ALX-746-251-T050 | 50 tests | 128.00 USD |  |
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| Sequence: | 5’-tttagggttagggttagggttaggg-3’(lower case letters indicate phosphorothioate linkage). |
| MW: | 8288 (ammonium salt) |
| Source: | Synthetic. |
| Quantity: | 24nmol (~200µg). Sufficient for ~50 cellular inhibition assays. Working concentration depends upon concentration, type and MW of stimulating CpG ODN, the cellular system and read-out used. Titrate at 3-10 fold molar excess for inhibition. |
| Formulation: | Lyophilized. Sterile. |
| Endotoxin Content: | <0.002EU/µg |
| Reconstitution: | For a 100µM stock solution, dissolve the total vial content in 240µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving. |
| Shipping: | Ambient Temperature |
| Long Term Storage: | +4°C |
| Use/Stability: | Aqueous stock solution is stable for 1 day when stored at +4°C. |
| Handling: | Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
| Scientific Background: | Several groups have studied the sequence requirements, specificity, signalling pathways and kinetics of the TLR (Toll-like receptor) 9 suppression by ‘inhibitory DNA motifs’, which led to a revised classification of iODNs, that is independent of the previously thought species preference. Class I: G-stretch ODNs: TLR9-specific competitors, some iODNs may also affect TLR7 and TLR8 signaling Class II: ODNs with telomeric repeats: TLR-independent inhibitors of STAT signalling (cellular uptake via an “ODN receptor”?) Class III: Inhibitors of DNA uptake in a sequence independent manner Class IV: Long phosphorothioate ODNs as direct competitors of TLR9 signaling in a sequence independent manner Slightly modified phosphodiester versions of the most potent inhibitory ODNs were also able to profoundly block the immune activation of macrophages and proved to be valuable tools for in vivo use in experimental animal models of inflammatory and auto-immune diseases. |
| Technical Info/Product Notes: | Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008). For active CpG compounds, please see Prod. No. ALX-746-005. |
| Regulatory Status: | RUO - Research Use Only |
ODN 2216 (TLRGRADE®) (synthetic) |
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| TLR9 ligand | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-005-C100 | 100 µg | 217.00 USD | |
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ODN 2006 (TLRGRADE®) (synthetic) |
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| TLR9 ligand | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-006-C100 | 100 µg | 233.00 USD | |
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ODN 2395 (TLRGRADE®) (synthetic) |
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| TLR9 ligand | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-020-C100 | 100 µg | 256.00 USD | |
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G-type-iODN (class I) (endotoxin-free) (synthetic) |
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| TLR9 inhibitor | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-252-C100 | 100 µg | 199.00 USD | |
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Dual-iODN (class I) (endotoxin-free) (synthetic) |
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| Inhibitor of TLR7 and TLR9 | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-255-C100 | 100 µg | 160.00 USD | |
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Neutral-ODN (Control for iODNs) (endotoxin-free) (synthetic) |
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| Inactive (neutral) control | ||||||
| Synthetic. | Print as PDF | ||||||
| ALX-746-256-C100 | 100 µg | 174.00 USD | |
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ddWater (endotoxin-free) (sterile) |
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| 7732-18-5 | Print as PDF | ||||||
| ALX-505-008-LD15 | 1.5 ml | 50.00 USD | |
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PBS (endotoxin-free) (sterile) |
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| Print as PDF | ||||||
| ALX-505-007-LD15 | 1.5 ml | 67.00 USD | |
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| ALX-505-007-L010 | 10 ml | 132.00 USD | |
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