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ODN 2395 (TLRgrade™) (synthetic) (BULK)

TLR9 ligand
 
ALX-746-070-M001 1 mg 533.00 USD
Do you need bulk/larger quantities?
 
For a smaller pack size, please see Prod. No. ALX-746-020.
CpG oligodeoxynucleotide (Type C) with phosphorothioate backbone. Specific ligand for human and mouse TLR9 (Toll-like receptor 9).

Product Specification

Sequence:5’-tcgtcgttttcggcgcgcgccg-3’(lower case letters indicate phosphorothioate linkage).
 
MW:7068 (ammonium salt)
 
Source:Synthetic.
 
Quantity:143nmol (~1'000µg).
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested - TLRgrade™.
 
Endotoxin Content:<0.002 EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 100µM stock solution, dissolve the total vial content in 1430µl endotoxin-free ddWater (Prod. No. ALX-505-008; included) or endotoxin-free PBS (Prod. No. ALX-505-007; order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see Prod. No. ALX-746-256.
 

General Literature References

Combined treatment of CpG-oligodeoxynucleotide with Nutlin-3 induces strong immune stimulation coupled to cytotoxicity in B-chronic lymphocytic leukemic (B-CLL) cells: P. Secchiero, et al.; J. Leukoc. Biol. 83, 434 (2008), Abstract;
Eosinophils induce DC maturation, regulating immunity: R. Lotfi & M.T. Lotze; J. Leukoc. Biol. 83, 456 (2008), Abstract;

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RELATED PRODUCTS

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TLR
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