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ODN 1585 (TLRgrade™) (synthetic) (BULK)

TLR9 ligand
 
ALX-746-053-M001 1 mg 533.00 USD
Do you need bulk/larger quantities?
 
For a smaller pack size, please see Prod. No. ALX-746-003.
CpG oligodeoxynucleotide (Type A) optimized for NK cell activation with mixed phosphodiester/phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).

Product Specification

Sequence:5’-ggGGTCAACGTTGAgggggg-3’(lower case letters indicate phosphorothioate linkage).
 
MW:6643 (ammonium salt)
 
Source:Synthetic.
 
Quantity:150nmol (~1'000µg).
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested - TLRgrade™.
 
Endotoxin Content:<0.002EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 100µM stock solution, dissolve the total vial content in 1'500µl endotoxin-free ddWater (Prod. No. ALX-505-008) or endotoxin-free PBS (Prod. No. ALX-505-007). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see Prod. No. ALX-746-203.
 

General Literature References

Induction of protective immunity against MHC class I-deficient, HPV16-associated tumours with peptide and dendritic cell-based vaccines: M. Reinis, et al.; Int. J. Oncol. 36, 545 (2010), Abstract;
Divergent therapeutic and immunologic effects of oligodeoxynucleotides with distinct CpG motifs: Z.K. Ballas, et al.; J. Immunol. 167, 4878 (2001), Abstract;
Interleukin-12- and gamma interferon-dependent protection against malaria conferred by CpG oligodeoxynucleotide in mice: R.A. Gramzinski, et al.; Infect. Immun. 69, 1643 (2001), Abstract;

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