Poly(deoxyadenosine:deoxythymidine) (endotoxin-free) (synthetic)

IFN activator



ALX-746-022-C050	50 µg	181.00 USD

Strong IFN inducer, independent of IRF3.

Product Specification

Endotoxin Content:	<0.0002EU/µg (LAL test; BioWhittaker)

Quantity:	Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 0.025-0.25µg/200µl.

Formulation:	Lyophilized. Sterile.

Source/Host:	Synthetic.

Reconstitution:	For a 250µg/ml stock solution, dissolve the total vial content in 200µl endotoxin-free buffer or water.

Long Term Storage:	+4°C

Use/Stability:	Aqueous stock solution is stable for 1 day when stored at +4°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Protect from light.

Background / Technical Information:	For in vitro stimulation we recommend the following procedure: 1. Bone marrow-derived mouse dendritic cells (BM mDC) are cultured 8x10⁴/100µl in RPMI containing 10% FCS over night at 5% CO₂ and 37°C. 2. The medium is removed and replaced by 100µl OPTI MEM (Invitrogen) and subsequently the transfection mixture is added: 25µl OPTI MEM containing 0.025 to 0.25 Poly(dA:dT) + 25µl OPTI MEM containing 0.5µl Lipofectamine 2000 (Invitrogen) or alternative transfection agents. 3. Over night stimulation at 5% CO₂ and 37°C. 4. Measurement of IFN-α/β in culture supernatants.

General Literature References

Cytosolic DNA recognition for triggering innate immune responses: A. Takaoka & T. Taniguchi; Adv. Drug Deliv. Rev. 60, 847 (2008), Abstract;

Intracellular pattern-recognition receptors : C. Dostert, et al.; Adv. Drug Deliv. Rev. 60, 830 (2008), Abstract;

The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response: D.A. Muruve, et al.; Nature 452, 103 (2008), Abstract;

DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response : A. Takaoka, et al.; Nature 448, 501 (2007), Abstract;