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Polyinosinic-polycytidylic acid . potassium salt (TLRgrade™) (synthetic)

Activator for TLR3 and MDA5
 
ALX-746-021-M002 2 mg 118.00 USD
 
ALX-746-021-M005 5 mg 155.00 USD
Do you need bulk/larger quantities?
 
Specific activator for TLR3 (Toll-like receptor 3) and MDA5/Helicard.

Product Specification

Alternative Name:poly(I:C) . K
 
Formula:(C10H10N4NaO7P)x • (C9H11N3NaO7P)x
 
Source:Synthetic.
 
CAS:31852-29-6
 
Quantity:Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml.
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested: TLRgrade™.
 
Endotoxin Content:<0.002EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
746-021 invitro
Figure 1: Poly(I:C) . K (TLRgrade™) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in murine BM-derived macrophages. Method: Poly(I:C) was added to bone marrow-derived murine macrophages in a 96-well plate at the indicated concentrations, cell supernatants were harvested after 24 hours and IFN α/β levels analyzed by cytokine ELISA.
746-021 invivo
Figure 2: Poly(I:C) . K (TLRgrade™) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in mouse in vivo. Method: Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.
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746-021 invitro 746-021 invivo

Product Literature References

Metabolic sialic acid blockade lowers the activation threshold of moDCs for TLR stimulation: C. Büll, et al.; Immunol. Cell Biol. (2016), Abstract;
Enterovirus-Infected β-Cells Induce Distinct Response Patterns in BDCA1+ and BDCA3+ Human Dendritic Cells: B. M. Schulte, et al.; PLoS One 10, e0121670 (2015), Application(s): Cell Culture, Abstract; Full Text
CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;
Activation of murine macrophages via TLR2 and TLR4 is negatively regulated by a Lyn/PI3K module and promoted by SHIP1: S. Keck, et al.; J. Immunol. 184, 5809 (2010), Abstract; Full Text
Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol. 83, 1118 (2008), Abstract;
Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006), Abstract;
The dsRNA binding site of human Toll-like receptor 3: J.K. Bell, et al.; PNAS 103, 8792 (2006), Abstract;
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003), Abstract;

General Literature References

Noncanonical inflammasome activation by intracellular LPS independent of TLR4.: N. Kayagaki, et al.; Science 341, 1246 (2013), Abstract;

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