Polyinosinic-polycytidylic acid . potassium salt (TLRgrade™) (synthetic)

Activator for TLR3 and MDA5



ALX-746-021-M002	2 mg	163.00 USD

ALX-746-021-M005	5 mg	319.00 USD

Specific activator for TLR3 (Toll-like receptor 3) and MDA5/Helicard.

Product Specification

Alternative Name:	poly(I:C) . K (TLRgrade™) (synthetic)

Purity Detail:	Activity and endotoxin tested: TLRgrade™.

Endotoxin Content:	<0.002EU/µg (LAL test; BioWhittaker)

Formula:	(C₁₀H₁₀N₄NaO₇P)_x • (C₉H₁₁N₃NaO₇P)_x

Quantity:	Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml.

Formulation:	Lyophilized. Sterile.

CAS:	31852-29-6

Source/Host:	Synthetic.

Reconstitution:	For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.

Long Term Storage:	+4°C

Use/Stability:	Aqueous stock solution is stable for 1 day when stored at +4°C.

Handling:	After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Protect from light.

Figure 1: Poly(I:C) . K (TLRgrade™) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in murine BM-derived macrophages. Method: Poly(I:C) was added to bone marrow-derived murine macrophages in a 96-well plate at the indicated concentrations, cell supernatants were harvested after 24 hours and IFN α/β levels analyzed by cytokine ELISA.

Figure 2: Poly(I:C) . K (TLRgrade™) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in mouse in vivo. Method: Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.

Please mouse over

Product Literature References

CD14 and TRIF govern distinct responsiveness and responses in mouse microglial TLR4 challenges by structural variants of LPS: T. Regen, et al.; Brain Behav. Immun. 25, 957 (2011), Abstract;

Activation of murine macrophages via TLR2 and TLR4 is negatively regulated by a Lyn/PI3K module and promoted by SHIP1: S. Keck, et al.; J. Immunol. 184, 5809 (2010), Abstract; Full Text

Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity: M. Peiser, et al.; J. Leukoc. Biol. 83, 1118 (2008), Abstract;

Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses: H. Kato, et al.; Nature 441, 101 (2006), Abstract;

The dsRNA binding site of human Toll-like receptor 3: J.K. Bell, et al.; PNAS 103, 8792 (2006), Abstract;

Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003), Abstract;