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ODN 2006 (TLRgrade™) (synthetic)

TLR9 ligand
 
ALX-746-006-C100 100 µg 178.00 USD
Do you need bulk/larger quantities?
 
For a larger pack size, please see Prod. No. ALX-746-056.
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for human and mouse TLR9 (Toll-like receptor 9).

Product Specification

Sequence:5’-tcgtcgttttgtcgttttgtcgtt-3’(lower case letters indicate phosphorothioate linkage).
 
MW:7720 (ammonium salt)
 
Source:Synthetic.
 
Quantity:12.5nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested - TLRgrade™.
 
Endotoxin Content:<0.002 EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 100µM stock solution, dissolve the total vial content in 125µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see Prod. No. ALX-746-206.
 

Product Literature References

TLR9-signaling is required for turning retinoic acid into a potent stimulator of RP105 (CD180)-mediated proliferation and IgG synthesis in human memory B cells: A. Eriksen, et al.; Cell. Immunol. 279, 87 (2012), Application(s): FLOW of human B cells, Abstract;

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