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ODN 2216 (TLRGRADE™) (synthetic)

TLR9 ligand
 
ALX-746-005-C100 100 µg 173.00 USD
Do you need bulk/larger quantities?
 
For a larger pack size, please see Prod. No. ALX-746-055.
CpG oligodeoxynucleotide (Type A) with mixed phosphodiester/phosphorothioate backbone. Specific ligand for human TLR9 (Toll-like receptor 9).

Product Specification

Sequence:5’-ggGGGACGATCGTCgggggg-3’ (lower case letters indicate phosphorothioate linkage).
 
MW:6644 (ammonium salt)
 
Source:Synthetic.
 
Quantity:15.1 nmol
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested - TLRGRADE™.
 
Endotoxin Content:<0.002 EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 100µM stock solution, dissolve the total vial content in 151µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For the inactive control compound, please see Prod. No. ALX-746-205.
 

Product Literature References

CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro: S. Rothenfusser, et al.; Blood 103, 2162 (2004), Abstract; Full Text

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