For a larger pack size, please see Prod. No. ALX-746-055
CpG oligodeoxynucleotide (Type A) with mixed phosphodiester/phosphorothioate backbone. Specific ligand for human TLR9 (Toll-like receptor 9).
|Sequence:||5’-ggGGGACGATCGTCgggggg-3’ (lower case letters indicate phosphorothioate linkage).|
|MW:||6644 (ammonium salt)|
|Purity Detail:||Activity and endotoxin tested - TLRgrade™.|
|Endotoxin Content:||<0.002 EU/µg (LAL test; BioWhittaker)|
|Reconstitution:||For a 100µM stock solution, dissolve the total vial content in 151µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure:|
- Add 50% of the solvent and let dissolve for 10 min.
- Add remaining 50% of the solvent and mix thoroughly.
- Moderate warming may aid dissolving.
|Long Term Storage:||+4°C|
|Use/Stability:||Aqueous stock solution is stable for 1 day when stored at +4°C.|
|Handling:||Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.|
|Technical Info/Product Notes:||Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).|
For the inactive control compound, please see Prod. No. ALX-746-205.
Product Literature References
CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro
: S. Rothenfusser, et al.; Blood 103
, 2162 (2004), Abstract
; Full Text