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ODN 1826 (TLRgrade™) (synthetic)

TLR9 ligand
 
ALX-746-002-C100 100 µg 147.00 USD
Do you need bulk/larger quantities?
 
For a larger pack size, please see Prod. No. ALX-746-052.
CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for mouse TLR9 (Toll-like receptor 9).

Product Specification

Alternative Name:CpG-B
 
Sequence:5’-tccatgacgttcctgacgtt-3’ (lower case letters indicate phosphorothioate linkage).
 
MW:6383 (ammonium salt)
 
Source:Synthetic.
 
Quantity:15nmol (~96µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
 
Formulation:Lyophilized. Sterile.
 
Purity Detail:Activity and endotoxin tested - TLRgrade™.
 
Endotoxin Content:<0.002EU/µg (LAL test; BioWhittaker)
 
Reconstitution:For a 100µM stock solution, dissolve the total vial content in 150µl endotoxin-free water (included) or PBS (to order separately).To obtain optimal dissolving we recommend the following procedure:- Add 50% of the solvent and let dissolve for 10 min.- Add remaining 50% of the solvent and mix thoroughly.- Moderate warming may aid dissolving.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Aqueous stock solution is stable for 1 day when stored at +4°C.
 
Handling:Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).
For inactive control compound, please see Prod. No. ALX-746-201.
 
746-002 1
Figure 1: TLR9-dependent activation of mouse macrophages by ODN 1826 (TLRgrade™) (synthetic) (Prod. No. ALX-746-002). Method: Culture of 5x105 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell activation was measured by IL12-p40 induction. Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No. ALX-746-201) activates wild type mouse macrophages. Activation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).
746-002 2
Figure 2: TLR9-dependent mitogenic activity of ODN 1826 (TLRgrade™) (synthetic) (Prod. No. ALX-746-002) in mouse splenocytes. Method: Culture of 5x105 cells per 200µl well was performed in serum-free conditions after addition of ODNs at the indicated concentrations. Cell proliferation was analyzed after 72 hours with a 16 hours3H-thymidine pulse.Only CpG ODN 1826 (Prod. No. ALX-746-002), but not the corresponding GpC control ODN 1982 (Prod. No. ALX-746-201) induces proliferation of wild type mouse splenocytes. Induction of cell proliferation is absolutely dependent on the presence of TLR9 as addressed with cells derived from TLR9-deficient mice. Note the logarithmic scale for the x and y-axis and the high activity of the active ODNs: max. stimulation is already achieved with 0.010-0.100nmol per 200µl well (10-100nM, corresponding to ~0.5-5ug/ml).
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Product Literature References

Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes: Y. Kameda, et al.; Biochem. Biophys. Res. Commun. 480, 215 (2016), Application(s): Assay for production of interferon γ (IFN-γ), spleen cells, Abstract;
Enhancing radiosensitivity of human pulmonary adenocarcinoma cell line A549 by CpG ODN1826: R. Li, et al.; Cancer Biother. Radiopharm. 26, 69 (2011), Abstract;
The role of intermediary domain of MyD88 in cell activation and therapeutic inhibition of TLRs: M. Avbelj, et al.; J. Immunol. 187, 2394 (2011), Abstract; Full Text
Cutting edge: the mechanism of invariant NKT cell responses to viral danger signals: A.J. Tyznik, et al.; J. Immunol. 181, 4452 (2008), Abstract; Full Text

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