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Isolectin B4 (Bandeiraea simplicifolia), (FITC conjugate)

 
ALX-650-001F-MC05 0.5 mg FITC 149.00 USD
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Product Specification

Alternative Name:Isolectin B4 (Griffonia simplicifolia), BSI-B4
 
Source:Isolated from Griffonia (Bandeiraea) simplicifolia seeds.
 
Concentration:1mg/ml
 
Formulation:Liquid. In 10mM HEPES, pH 7.5, containing 0.15M sodium chloride, 0.08% sodium azide and 0.1mM Ca2+.
 
Quality Control:Free Fluorescein
F/P (molar)
Active Conjugate
 
Specificity:Binds to α-(1,3) galactosyl galactoside epitopes.
 
Activity:Agglutinates blood group B glutaraldehyde-fixed human red blood cells at a concentration of ~2µg/ml. No agglutination of blood group A, glutaraldehyde-fixed human red blood cells occurred at concentrations up to ~100µg/ml.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Use/Stability:Prepare fresh dilutions prior to each application.
 
Handling:Protect from light.
 
Scientific Background: BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4.
BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
 

Product Literature References

Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer: L. Bao, et al.; Sci. China Life Sci. 57, 263 (2014), Abstract; Full Text
Fine mapping of the 1p36 deletion syndrome identifies mutation of PRDM16 as a cause of cardiomyopathy: A.K. Arndt, et al.; Am. J. Hum. Genet. 93, 67 (2013), Application(s): IHC using embryonic mouse heart, Abstract; Full Text
Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases: J. Hauschild, et al.; PNAS 108, 12013 (2011), Application(s): Used for FC with porcine cells, Abstract; Full Text
Isoliquiritigenin from licorice root suppressed neovascularisation in experimental ocular angiogenesis models: V. Jhanji, et al.; Br. J. Ophthalmol. 95, 1309 (2011), Abstract;
Isolectins I-A and I-B of Griffonia (Bandeiraea) simplicifolia. Crystal structure of metal-free GS I-B(4) and molecular basis for metal binding and monosaccharide specificity: J. Lescar, et al.; J. Biol. Chem. 277, 6608 (2002), Abstract; Full Text

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