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α-Latrotoxin

Neurotoxin
 
ALX-630-027-C040 40 µg 354.00 USD
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Causes massive neurotransmitter release from a wide variety of central and peripheral synaptic junctions of vertebrates using Ca2+-dependent and Ca2+-independent pathways. A useful pharmacological tool in the studies of synaptic vesicles exocytosis of different neutrotransmitters.

Product Specification

MW:~130kDa.
 
Source:Isolated from Latrodectus tredecimguttatus.
 
CAS:65988-34-3
 
RTECS:OE9020000
 
UniProt ID:P23631
 
Quantity:Determined by Lowry and Pierce method.
 
Formulation:Lyophilized.
 
Purity:≥97%
 
Purity Detail:Migrates as one distinct band when run on a 5-25% gradient polyacrylamide gel according to the method of Laemmli (Nature 227, 680 (1970)).
 
Quality Control:Bioassay: Test of the ability to stimulate neurotransmitter release in both Ca2+-free and Ca2+-containing media according to Valtorta. Effective concentration: 100pM-1nM.
 
Reconstitution:Reconstitute in 0.5ml of distilled cold water. Stir very gently. Do not shake or vortex. Add 0.5 ml glycerol. Stock solution received contains 50% glycerol and 300nM α-latrotoxin.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:Stock solution is stable for 1 week when stored at +4°C or for at least one year at -20°C.
 
Technical Info/Product Notes:Attention: For best results, only proceed according to the given instructions. Dissolution and/or storage of this toxin contrary to these instructions may affect the stability and biological activity of this compound.
 

Product Literature References

Alpha-latrotoxin induces exocytosis by inhibition of voltage-dependent K+ channels and by stimulation of L-type Ca2+ channels via latrophilin in beta-cells: S. Lajus, et al.; J. Biol. Chem. 281, 5522 (2006), Abstract;
alpha-Latrotoxin and its receptors: neurexins and CIRL/latrophilins: T.C. Sudhof; Annu. Rev. Neurosci. 24, 933 (2001), (Review), Abstract;
Mechanisms of alpha-latrotoxin action: A. W. Henkel & S. Sankaranarayanan; Cell Tissue Res. 296, 229 (1999), (Review), Abstract;
Alpha-latrotoxin stimulates glutamate release from cortical astrocytes in cell culture: V. Parpura, et al.; FEBS Lett. 360, 266 (1995), Abstract;
GTP cleavage by the small GTP-binding protein Rab3A is associated with exocytosis of synaptic vesicles induced by alpha-latrotoxin: B. Stahl, et al.; J. Biol. Chem. 269, 24770 (1994), Abstract; Full Text
The effect of alpha-latrotoxin on a synaptic connection between identified neurons in the brain of the mollusc Helix pomatia L: O.N. Osipenko, et al.; Toxicon 31, 1123 (1993), Abstract;
Synaptophysin (p38) at the frog neuromuscular junction: its incorporation into the axolemma and recycling after intense quantal secretion: F. Valtorta, et al.; J. Cell. Biol. 107, 2717 (1988), Abstract;
Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus): A. Grasso; Biochim. Biophys. Acta 439, 406 (1976), Abstract;
Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions: N. Frontali, et al.; J. Cell. Biol. 68, 462 (1976), Abstract;

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